Prominent expression of glial cell line-derived neurotrophic factor in human skeletal muscle

被引:1
作者
Suzuki, H
Hase, A
Miyata, Y
Arahata, K
Akazawa, C
机构
[1] Natl Inst Neurosci, NCNP, Dept Neuromuscular Res, Tokyo 1878502, Japan
[2] Nippon Med Sch, Dept Pharmacol, Tokyo 1138602, Japan
关键词
motor neuron; neuromuscular junction; axon; immunohistochemistry;
D O I
暂无
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Glial cell Line-derived neurotrophic factor (GDNF) has been shown to exert neurotrophic effects on motor neurons as well as mesencephalic dopaminergic neurons. Because GDNF promotes survival of motor neurons in vivo and in vitro and rescues motor neurons from naturally occurring cell death, the potential use of GDNF for treatment of motor neuron diseases has been a major focus of recent research. The expression of GDNF in humans, however, has not been fully examined. In the present study, we examined the expression of GDNF in adult human muscle by Northern blot, reverse transcriptase polymerase chain reaction (RT-PCR), and immunohistochemical analyses to address physiological roles of GDNF in humans. Northern blot analysis demonstrated high expression of GDNF mRNA in human skeletal muscle when compared to that of mouse. Intense GDNF immunoreactivity was observed in the vicinity of plasma membranes of skeletal muscle, particularly at neuromuscular junctions. GDNF immunoreactivity was also observed within the axons and surrounding Schwann cells of peripheral nerves. However, RT-PCR detected expression of GDNF mRNA only in skeletal muscle, and not within the anterior horn cells of human spinal cord. These results suggest that GDNF is produced by skeletal muscle and taken up at the nerve terminals for retrograde transport by axons. Thus, GDNF in human skeletal muscle may be involved in promoting motor neuron survival as a target-derived neurotrophic factor. J. Comp. Neurol. 402:303-312, 1998. (C) 1998 Wiley-Liss, Inc.
引用
收藏
页码:303 / 312
页数:10
相关论文
共 50 条
[41]   Expression of multiple nebulin isoforms in human skeletal muscle and brain [J].
Laitila, Jenni ;
Hanif, Mubashir ;
Paetau, Anders ;
Hujanen, Sari ;
Keto, Joni ;
Somervuo, Panu ;
Huovinen, Sanna ;
Udd, Bjarne ;
Wallgren-Pettersson, Carina ;
Auvinen, Petri ;
Hackman, Peter ;
Pelin, Katarina .
MUSCLE & NERVE, 2012, 46 (05) :730-737
[42]   Expression of sialic acids in human adult skeletal muscle tissue [J].
Marini, Mirca ;
Ambrosini, Stefano ;
Sarchielli, Erica ;
Thyrion, Giorgia Donata Zappoli ;
Bonaccini, Laura ;
Vannelli, Gabriella Barbara ;
Sgambati, Eleonora .
ACTA HISTOCHEMICA, 2014, 116 (05) :926-935
[43]   Gene and protein expression of brain-derived neurotrophic factor and TrkB in bone and cartilage [J].
Yamashiro, T ;
Fukunaga, T ;
Yamashita, K ;
Kobashi, N ;
Takano-Yamamoto, T .
BONE, 2001, 28 (04) :404-409
[44]   Development of an in vitro potency assay for human skeletal muscle derived cells [J].
Thurner, Marco ;
Asim, Faheem ;
Garczarczyk-Asim, Dorota ;
Janke, Katrin ;
Deutsch, Martin ;
Margreiter, Eva ;
Troppmair, Jakob ;
Marksteiner, Rainer .
PLOS ONE, 2018, 13 (03)
[45]   The expression of platelet-derived endothelial cell growth factor in human bladder cancer [J].
Sawase, K ;
Nomata, K ;
Kanetake, H ;
Saito, Y .
CANCER LETTERS, 1998, 130 (1-2) :35-41
[46]   CLONING OF THE ALPHA-COMPONENT OF THE CHICK CILIARY NEUROTROPHIC FACTOR-RECEPTOR - DEVELOPMENTAL EXPRESSION AND DOWN-REGULATION IN DENERVATED SKELETAL-MUSCLE [J].
IP, FCF ;
FU, AKY ;
TSIM, KWK ;
IP, NY .
JOURNAL OF NEUROCHEMISTRY, 1995, 65 (06) :2393-2400
[47]   Expression of brain-derived neurotrophic factor protein in the adult rat central nervous system [J].
Yan, Q ;
Rosenfeld, RD ;
Matheson, CR ;
Hawkins, N ;
Lopez, OT ;
Bennett, L ;
Welcher, AA .
NEUROSCIENCE, 1997, 78 (02) :431-448
[48]   Expression disparity of brain-derived neurotrophic factor immunoreactivity and mRNA in ischemic hippocampal neurons [J].
Lee, TH ;
Kato, H ;
Chen, ST ;
Kogure, K ;
Itoyama, Y .
NEUROREPORT, 2002, 13 (17) :2271-2275
[49]   HUMAN CARDIAC AND SKELETAL-MUSCLE SPECTRINS - DIFFERENTIAL EXPRESSION AND LOCALIZATION [J].
VYBIRAL, T ;
WINKELMANN, JC ;
ROBERTS, R ;
JOE, EH ;
CASEY, DL ;
WILLIAMS, JK ;
EPSTEIN, HF .
CELL MOTILITY AND THE CYTOSKELETON, 1992, 21 (04) :293-304
[50]   Expression of PC-cell-derived growth factor in benign and malignant human breast epithelium [J].
Serrero, G ;
Ioffe, OB .
HUMAN PATHOLOGY, 2003, 34 (11) :1148-1154