Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells

被引：51

作者：

Cronholm, Pontus ^{[1
]}

Midander, Klara ^{[2
]}

Karlsson, Hanna L. ^{[1
]}

Elihn, Karine ^{[3
]}

Wallinder, Inger Odnevall ^{[2
]}

Moller, Lennart ^{[1
]}

机构：

[1] Karolinska Inst, Novum, Unit Analyt Toxicol, Dept Biosci & Nutr, Stockholm, Sweden

[2] Royal Inst Technol, Sch Chem Sci & Engn, Dept Chem, Div Surface & Corros Sci, Stockholm, Sweden

[3] Stockholm Univ, Dept Appl Environm Sci, Workpl Aerosols Res Grp, S-10691 Stockholm, Sweden

来源：

NANOTOXICOLOGY | 2011年 / 5卷 / 02期

基金：

瑞典研究理事会;

关键词：

Nanoparticles; nanomedicine; nanotoxicology; particle toxicology; METAL-OXIDE NANOPARTICLES; IN-VITRO; PARTICLES; SIZE; AGGLOMERATION; GENOTOXICITY; DISPERSION;

D O I：

10.3109/17435390.2010.536268

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Different methodological settings can influence particle characteristics and toxicity in nanotoxicology. The aim of this study was to investigate how serum proteins and sonication of Cu nanoparticle suspensions influence the properties of the nanoparticles and toxicological responses on human lung epithelial cells. This was investigated by using methods for particle characterization (photon correlation spectroscopy and TEM) and Cu release (atomic absorption spectroscopy) in combination with assays for analyzing cell toxicity (MTT-, trypan blue- and Comet assay). The results showed that sonication of Cu nanoparticles caused decreased cell viability and increased Cu release compared to non-sonicated particles. Furthermore, serum in the cell medium resulted in less particle agglomeration and increased Cu release compared with medium without serum, but no clear difference in toxicity was detected. Few cells showed intracellular Cu nanoparticles due to fast release/dissolution processes of Cu. In conclusion; sonication can affect the toxicity of nanoparticles.

引用

页码：269 / 281

页数：13

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