Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women

被引:6
作者
Kato, Satsuki [1 ]
Nagasawa, Toshiyuki [2 ]
Uehara, Osamu [3 ]
Shimizu, Shintaro [1 ]
Sugiyama, Nodoka [1 ]
Hasegawa-Nakamura, Kozue [4 ]
Noguchi, Kazuyuki [4 ]
Hatae, Masayuki [5 ]
Kakinoki, Hiroshige [5 ]
Furuichi, Yasushi [1 ]
机构
[1] Hlth Sci Univ Hokkaido, Sch Dent, Dept Oral Rehabil, Div Periodontol & Endodontol, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan
[2] Hlth Sci Univ Hokkaido, Sch Dent, Dept Integrated Dent Educ, Div Adv Clin Educ, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan
[3] Hlth Sci Univ Hokkaido, Sch Dent, Dept Oral Growth & Dev, Div Dis Control & Mol Epidemiol, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan
[4] Kagoshima Univ, Grad Sch Med & Dent Sci, Adv Therapeut Course, Dept Periodontol Field Oral & Maxillofacial Rehab, 8-35-1 Sakuragaoka, Kagoshima 8908532, Japan
[5] Kakinoki Hosp, 8-13 Kajiyamachi, Kagoshima 8920846, Japan
关键词
Pregnancy; Salivary microbiota; Estradiol; Progesterone; Bifidobacterium; SEX STEROID-HORMONES; PERIODONTAL HEALTH; PRETERM BIRTH; PORPHYROMONAS-GINGIVALIS; GUT MICROBIOTA; DENTAL-CARIES; BACTERIA; IMPACT; PCR; PROGESTERONE;
D O I
10.1186/s12903-022-02293-0
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background The establishment of symbiotic microbiota in pregnant women is important for both the mother and her offspring. Little is known about the salivary symbiotic bacteria in pregnancy, and analysis of composition of microbiome (ANCOM) is useful to detect small differences in the number of bacteria. The aim of this study was to investigate the differences in the salivary bacteria between healthy pregnant and non-pregnant women using ANCOM. Methods Unstimulated saliva samples were collected from 35 healthy pregnant women at 35 weeks gestation and 30 healthy non-pregnant women during menstruation. All participants underwent a periodontal examination. Estradiol and progesterone levels were examined by enzyme-linked immunosorbent assay. DNA extracted from the saliva was assessed by 16S ribosomal RNA amplicon sequencing and real-time PCR. Results Salivary estradiol and progesterone levels were significantly increased in pregnant women. The alpha and beta diversities were higher in pregnant women than in non-pregnant women. The largest effect size difference noted when the microbiota of the pregnant and non-pregnant women were analyzed was that for Bifidobacteriales. Levels of Bifidobacterium dentium, but not of Bifidobacterium adolescentis, were significantly increased in pregnant women, and the levels were significantly correlated with progesterone concentration. Conclusion The results suggest that Bifidobacterium and progesterone levels are elevated in the saliva of healthy pregnant women compared with non-pregnant women.
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页数:13
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