Yeast surface display for protein engineering and characterization

被引：278
作者：
Gai, S. Annie
Wittrup, K. Dane
机构：
[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[2] MIT, Dept Biol Engn, Cambridge, MA 02139 USA
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2007年 / 17卷 / 04期
关键词：
D O I：
10.1016/j.sbi.2007.08.012
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.
引用
收藏
页码：467 / 473
页数：7
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