Chitooligosaccharide-induced activation of o-phenylenediamine oxidation by wheat seedlings in the presence of oxalic acid

被引：15

作者：

Khairullin, RM ^{[1
]}

Yarullina, LG ^{[1
]}

Troshina, NB ^{[1
]}

Akhmetova, IE ^{[1
]}

机构：

[1] Russian Acad Sci, Ufa Sci Ctr, Inst Biochem & Genet, Ufa 450054, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2001年 / 66卷 / 03期

关键词：

wheat seedlings; oxalic acid; oxalate oxidase; o-phenylenediamine; oxidation; chitooligosaccharides; induction;

D O I：

10.1023/A:1010247712723

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for determination of oxidation of phenolic compounds by intact wheat seedlings using omicron -phenylenediammine (OPD) was developed. The reaction is initiated by the addition of oxalic acid to the incubation medium. It is suggested that an endogenous peroxidase and hydrogen peroxide formed during oxidation of oxalic acid by endogenous oxalate oxidase are involved in OPD oxidation. Treatment of plants with chitooligosaccharides (1-10 mg/liter) with acetylation degree of 65% and molecular masses of 5-10 kD significantly activated OPD oxidation by wheat seedlings.

引用

页码：286 / 289

页数：4

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