Resonance Light Scattering Method for Study on the Interaction Between Fluorinated Surfactant Potassium Perfluorooctanesulfonate and DNA

被引：4
作者：
Li, Ling [1 ]
Pan, Quan [1 ]
Xu, Zu Shun [1 ]
Song, Gong Wu [1 ]
机构：
[1] Hubei Univ, Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Hubei Province, Peoples R China
来源：
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY | 2010年 / 31卷 / 12期
关键词：
Aggregation; DNA; fluorinated surfactant; resonance light-scattering; HSA SERUM ALBUMINS; NUCLEIC-ACIDS; BOVINE BSA; PROBE;
D O I：
10.1080/01932690903297256
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Resonance light-scattering (RLS) characteristics of the interactions between fluorinated surfactant potassium perfluorooctanesulfonate (PFOS) with calf thymus nucleic acid (ct DNA) were studied. After DNA was added, aggregation of PFOS on the molecular surface of DNA occurred in the pH 3.56.0, which resulted in an enhanced RLS peak at 370nm. The RLS intensity was found to be proportional to the concentration of DNA. The determination limit was 20.0g center dot L-1. UV-visible (UV-vis) spectra and infrared (IR) spectra both proved the binding changed the confromation of DNA.
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收藏
页码：1691 / 1696
页数：6
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