Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout

被引:19
作者
Declercq, Annelies Maria [1 ]
Chiers, Koen [2 ]
Van den Broeck, Wim [1 ]
Dewulf, Jeroen [3 ]
Eeckhaut, Venessa [2 ]
Cornelissen, Maria [4 ]
Bossier, Peter [5 ,6 ]
Haesebrouck, Freddy [2 ]
Decostere, Annemie [1 ]
机构
[1] Univ Ghent, Fac Vet Med, Dept Morphol, B-9820 Merelbeke, Belgium
[2] Univ Ghent, Dept Pathol Bacteriol & Poultry Dis, Fac Vet Med, B-9820 Merelbeke, Belgium
[3] Univ Ghent, Fac Vet Med, Dept Reprod Obstet & Herd Hlth, B-9820 Merelbeke, Belgium
[4] Univ Ghent, Fac Med & Hlth Sci, Dept Basic Med Sci, B-9000 Ghent, Belgium
[5] Univ Ghent, Lab Aquaculture, B-9000 Ghent, Belgium
[6] Artemia Reference Ctr, B-9000 Ghent, Belgium
关键词
OUTER-MEMBRANE VESICLES; FISH PATHOGEN; FLEXIBACTER-COLUMNARIS; TELEOSTEAN FISH; GRANULE CELLS; COMMON CARP; APOPTOSIS; STRAINS; DISEASE; MUCUS;
D O I
10.1186/s13567-015-0164-5
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The interactions of Flavobacterium columnare isolates of different virulence with the gills of carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum) were investigated. Both fish species were exposed to different high (HV) or low virulence (LV) isolates and sacrificed at seven predetermined times post-challenge. Histopathological and ultrastructural examination of carp and rainbow trout inoculated with the HV-isolate disclosed bacterial invasion and concomitant destruction of the gill tissue, gradually spreading from the filament tips towards the base, with outer membrane vesicles surrounding most bacterial cells. In carp, 5-10% of the fish inoculated with the LV-isolate became moribund and their gill tissue displayed the same features as described for the HV-isolate, albeit to a lesser degree. The bacterial numbers retrieved from the gill tissue were significantly higher for HV-compared to LV-isolate challenged carp and rainbow trout. TUNEL-stained and caspase-3-immunostained gill sections demonstrated significantly higher apoptotic cell counts in carp and rainbow trout challenged with the HV-isolate compared to control animals. Periodic acid-Schiff/alcian blue staining demonstrated a significantly higher total gill goblet cell count for HV- and LV-isolate challenged compared to control carp. Moreover, bacterial clusters were embedded in a neutral matrix while being encased by acid mucins, resembling biofilm formation. Eosinophilic granular cell counts were significantly higher in the HV-isolate compared to LV-isolate inoculated and control carp. The present data indicate a high colonization capacity, and the destructive and apoptotic-promoting features of the HV-isolate, and point towards important dynamic host mucin-F. columnare interactions warranting further research.
引用
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页数:16
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