Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding

被引：14

作者：

Kalabova, Dana ^{[1
,4
]}

Smidova, Aneta ^{[1
]}

Petrvalska, Olivia ^{[1
,2
]}

Alblova, Miroslava ^{[1
]}

Kosek, Dalibor ^{[1
,2
]}

Man, Petr ^{[3
]}

Obsil, Tomas ^{[1
,2
]}

Obsilova, Veronika ^{[1
]}

机构：

[1] Czech Acad Sci, Div BIOCEV, Dept Struct Biol Signaling Prot, Inst Physiol, Prumyslova 595, Vestec 25250, Czech Republic

[2] Charles Univ Prague, Fac Sci, Dept Phys & Macromol Chem, Prague 12843, Czech Republic

[3] Czech Acad Sci, Inst Microbiol, BIOCEV, Prumyslova 595, Vestec 25250, Czech Republic

[4] Charles Univ Prague, Fac Med 2, V Uvalu 84, Prague 15006, Czech Republic

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 493卷 / 02期

关键词：

Procaspase-2; 14-3-3; Protein-protein interaction; PhosphoryIation; Caspase-2; NUCLEAR-LOCALIZATION; CRYSTAL-STRUCTURE; STRUCTURAL BASIS; CASPASE-2; ACTIVATION; APOPTOSIS; PROTEIN; RECOGNITION; EXPRESSION; PRODOMAIN;

D O I：

10.1016/j.bbrc.2017.09.116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Procaspase-2 phosphorylation at several residues prevents its activation and blocks apoptosis. This process involves procaspase-2 phosphorylation at 5164 and its binding to the scaffolding protein 14-3-3. However, bioinformatics analysis has suggested that a second phosphoserine-containing motif may also be required for 14-3-3 binding. In this study, we show that human procaspase-2 interaction with 14-3-3 is governed by phosphorylation at both 5139 and 5164. Using biochemical and biophysical approaches, we show that doubly phosphorylated procaspase-2 and 14-3-3 form an equimolar complex with a dissociation constant in the nanomolar range. Furthermore, our data indicate that other regions of procaspase-2, in addition to phosphorylation motifs, may be involved in the interaction with 14-3-3. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：940 / 945

页数：6

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