Construction of artificial cyclic amide amidohydrolases using molecular imprinting technique

被引：4
|
作者：
Meng, ZH [1 ]
Yamazaki, T [1 ]
Sode, K [1 ]
机构：
[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan
来源：
BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 07期
基金：
日本学术振兴会;
关键词：
artificial enzyme; creatininase; cyclic amide amidohydrolase; hydantoinase; molecular imprinting;
D O I：
10.1007/s10529-005-2223-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A general molecular imprinting approach is proposed to synthesize artificial enzymes to mimic the family of cyclic amide amidohydrolases which share similar active site and catalytic mechanism. The artificial enzymes were constructed by co-polymerizing 4(5)-vinylimidazole-Co2+-methacrylic acid clusters with divinylbenzene micro-spheres in the presence of corresponding substrates. The artificial enzymes mimicked creatininase and hydantoinase by showing specific affinity towards the corresponding substrates in buffer. The artificial hydantoinase also showed specific affinity towards corresponding substrate in organic solvent, and catalyzed the hydrolysis of hydantoin.
引用
收藏
页码：471 / 475
页数：5
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