Characterization of protein conjugates using capillary electrophoresis

被引：4

作者：

Safi, Samir ^{[1
]}

Asfari, Zouhair ^{[1
]}

Hagege, Agnes ^{[1
]}

机构：

[1] Univ Strasbourg 1, CNRS, ECPM, IPHC DSA,UMR 7178,Lab Chim Analyt & Minerale, F-67087 Strasbourg, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1173卷 / 1-2期

关键词：

capillary electrophoresis; conjugation; hapten; bovine serum albumin; conjugate heterogeneity;

D O I：

10.1016/j.chroma.2007.10.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

With the aim of generating antibodies, a calix[4]arene-crown-6 was coupled to bovine serum albumin. For that purpose, a complete procedure to optimize and characterize the coupling of hydrophobic haptens based on capillary electrophoresis (CE) was developed. We demonstrated the existence of a polynomial relationship between the electrophoretic mobility (mu(ep)) and the hapten density. This correlation was used not only to study the coupling reaction in terms of optimization and kinetics but also to determine the average coupling molar ratio of any given conjugate. An estimation of the heterogeneity of these conjugates by simulation of experimental peaks was also proposed. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：159 / 164

页数：6

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