Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems

被引：17

作者：

Wang, Le ^{[1
,2
]}

Chen, Man ^{[1
,2
]}

Wu, Bo ^{[3
]}

Liu, Yi-Cheng ^{[1
,2
]}

Zhang, Guang-Feng ^{[1
,2
]}

Jiang, Li ^{[1
,2
]}

Xu, Xiu-Lan ^{[1
,2
]}

Zhao, Xing-Chun ^{[1
,2
]}

Ji, An-Quan ^{[1
,2
]}

Ye, Jian ^{[1
,2
]}

机构：

[1] Minist Publ Secur, Inst Forens Sci, Nat Engn Lab Forens Sci, Beijing 100038, Peoples R China

[2] Minist Publ Secur, Inst Forens Sci, MPS Key Lab Forens Genet, Beijing 100038, Peoples R China

[3] Shanxi Med Univ, Sch Forens Med, Taiyuan 030001, Shanxi, Peoples R China

来源：

JOURNAL OF FORENSIC SCIENCES | 2018年 / 63卷 / 06期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

forensic science; forensic genetics; next-generation sequencing; short tandem repeat; Ion S5 (TM) XL; Ion Chef (TM); STRAIT RAZOR; LOCI; IDENTIFICATION; KIT; MUTATIONS; VARIANTS; D12S391; D21S11; TOOL;

D O I：

10.1111/1556-4029.13767

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5 (TM) XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef (TM) and S5 (TM) XL systems using an early access 25-plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA. In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef (TM) and the Ion S5 (TM) XL systems provided an alternative and promising approach for forensic STR genotyping.

引用

页码：1692 / 1703

页数：12

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