Cellular internalization of fluorescent proteins via arginine-rich intracellular delivery peptide in plant cells

被引:87
|
作者
Chang, M
Chou, JC
Lee, HJ [1 ]
机构
[1] Natl Dong Hwa Univ, Inst Biotechnol, Hualien 974, Taiwan
[2] Natl Dong Hwa Univ, Dept Life Sci, Hualien 974, Taiwan
[3] Tzu Chi Univ, Inst Med Sci, Hualien 970, Taiwan
关键词
cellular internalization; green fluorescent protein; protein transduction; recombinant protein; transgenic plant;
D O I
10.1093/pcp/pci046
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The protein delivery across cellular membranes or compartments is limited by low biomembrane permeability because of the hydrophobic characteristics of cell membranes. Usually the delivery processes utilize passive protein channels or active transporters to overcome the membrane impediment. In this report, we demonstrate that arginine-rich intracellular delivery (AID) peptide is capable of efficiently delivering fused fluorescent proteins unpreferentially into different plant tissues of both tomato (a dicot plant) and onion (a monocot plant) in a fully bioactive form. Thus, cellular internalization via AID peptide can be a powerful tool characterized by its simplicity, non-invasion and high efficiency to express those bioactive proteins in planta or in plant cells in vivo. This novel method may alternatively provide broader applications of AID chimera in plants without the time-consuming transgenic approaches.
引用
收藏
页码:482 / 488
页数:7
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