Long non-coding RNA OIP5-AS1 promotes the progression of esophageal cancer by regulating miR-30a/VOPP1 expression

被引：9

作者：

Xu, Jiajun ^{[1
]}

Chen, Zhixi ^{[2
]}

Fang, Zheng ^{[1
]}

Chen, Shixiong ^{[1
]}

Guo, Ying ^{[1
]}

Liu, Xianfeng ^{[1
]}

Chen, Kai ^{[1
]}

Chen, Shengjia ^{[1
]}

机构：

[1] Nanchang Univ, Affiliated Jiujiang Hosp, Dept Cardiothorac Surg, 48 Taling Southern Rd, Jiujiang 332000, Jianxi, Peoples R China

[2] Gannan Med Univ, Coll Pharm, Ganzhou 341000, Jianxi, Peoples R China

来源：

ONCOLOGY LETTERS | 2021年 / 22卷 / 03期

基金：

美国国家科学基金会;

关键词：

esophageal cancer; opa-interacting protein 5 antisense transcript 1; microRNA-30a; vesicular overexpressed in cancer prosurvival protein 1; migration; invasion; VOPP1; IDENTIFICATION; TUMORIGENESIS; GROWTH; GENES;

D O I：

10.3892/ol.2021.12912

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Long non-coding RNAs (lncRNAs) serve an important role in the development of esophageal cancer (EC), which is the eighth most common type of cancer worldwide. lncRNA opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is associated with human malignancy. However, the biological roles of OIP5-AS1 in the development of EC remain unclear. In the present study, transfection was conducted, and reverse transcription-quantitative PCR and western blot analysis were used for the detection of mRNA and protein expression, respectively. Furthermore, dual-luciferase reporter and RNA immunoprecipitation assays were used to study the interaction between miRNA and lncRNA or genes. The results revealed that OIP5-AS1 expression in EC tissues and cultured EC cells was upregulated, microRNA-30a (miR-30a) expression was downregulated. OIP5-AS1-knockdown suppressed the proliferation, migration and invasion of EC9706 and EC109 cells. miR-30a was confirmed to interact with OIP5-AS1, and miR-30a-mimics transfection ameliorated the effects of OIP5-AS1 in EC cells. Vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) was verified as the direct target of miR-30a. VOPP1 expression was positively correlated with OIP5-AS1 expression in EC cells. Overexpression of VOPP1 ameliorated the negative effects of OIP5-AS1-knockdown on EC9706 and EC109 cells. In conclusion, OIP5-AS1 promoted the proliferation, migration and invasion of EC cells by increasing VOPP1 expression by sponging miR-30a.

引用

页数：9

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