S-Allylcysteine as an Inhibitor of Benzo(a)pyrene-Induced Precancerous Carcinogenesis in Human Lung Cells via Inhibiting Activation of Nuclear Factor-Kappa B

Oil-soluble organosulfur compounds in garlic are known for the anticancer effect. However, there are limited experimental studies to describe the effect of S-allylcysteine (SAC), a main water-soluble derivative of garlic, in carcinogenesis. This study investigates the prevention function of SAC on carcinogen benzo(a)pyrene (B(a)P)-induced precancerous activity in human lung cells (A549). A549 cells were either pretreated (PreTM) or concurrently treated (CoTM) with 1 mu M B(a)P and either 10 or 50 mu M SAC. The 50 mu M CoTM group inhibited B(a)P-induced cell proliferation by approximately 100%. The 50 mu M SAC CoTM and PreTM inhibited the B(a)P-induced G2/M phase shift by 119% and 100%, respectively. Furthermore, the SAC PreTM exhibited the potential to reduce the generation of reactive oxygen species (ROS) in cells relative to the B(a)P group by approximately 100%. The CoTM and PreTM elevated superoxide dismutase (SOD) by at least 70% compared with B(a)P group. In this study, we demonstrated that the mechanisms involved in the inhibitory role of SAC in B(a)P-induced carcinogenesis, including suppression of cell proliferation and DNA damage, cell cycle regulation, attenuation of ROS formation, increase of SOD activity, and inhibition of nuclear factor-kappa B (NF-kappa B) activity, which indicated that SAC is potentially a novel therapeutic candidate for the prevention and treatment of B(a)P-induced human lung cancer.