Plot-scale manipulations of organic matter inputs to soils correlate with shifts in microbial community composition in a lowland tropical rain forest

被引:223
|
作者
Nemergut, Diana R. [1 ,2 ]
Cleveland, Cory C. [3 ]
Wieder, William R. [1 ,4 ]
Washenberger, Christopher L. [1 ]
Townsend, Alan R. [1 ,4 ]
机构
[1] Univ Colorado, Inst Arct & Alpine Res, Boulder, CO 80309 USA
[2] Univ Colorado, Environm Studies Program, Boulder, CO 80309 USA
[3] Univ Montana, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA
[4] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA
来源
SOIL BIOLOGY & BIOCHEMISTRY | 2010年 / 42卷 / 12期
基金
美国国家科学基金会;
关键词
Soil microbial community; Lowland tropical rain forest; Soil carbon cycling; 16S rRNA gene sequencing; Pyrosequencing; Acidobacteria; Alphaproteobacteria; Archaea; CARBON-DIOXIDE LOSSES; EXTRACTION METHOD; DECOMPOSITION; DIVERSITY; NITROGEN; BIOMASS; MICROORGANISMS; RESPIRATION; INCREASES; ADDITIONS;
D O I
10.1016/j.soilbio.2010.08.011
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Little is known about the organisms responsible for decomposition in terrestrial ecosystems, or how variations in their relative abundance may influence soil carbon (C) cycling. Here, we altered organic matter in situ by manipulating both litter and throughfall inputs to tropical rain forest soils, and then used qPCR and error-corrected bar-coded pyrosequencing to investigate how the resulting changes in soil chemical properties affected microbial community structure. The plot-scale manipulations drove significant changes in microbial community composition: Acidobacteria were present in greater relative abundance in litter removal plots than in double-litter plots, while Alphaproteobacteria were found in higher relative abundance in double-litter and throughfall reduction plots than in control or litter removal plots. In addition, the bacterial:archaeal ratio was higher in double-litter than no-litter plots. The relative abundances of Actinobacteria, Alphaproteobacteria and Gammaproteobacteria were positively correlated with microbial biomass C and nitrogen (N), and soil N and C pools, while acidobacterial relative abundance was negatively correlated with these same factors. Bacterial:archaeal ratios were positively correlated with soil moisture, total soil C and N, extractable ammonium pools, and soil C:N ratios. Additionally, bacterial:archaeal ratios were positively related to the relative abundance of Actinobacteria, Gammaproteobacteria, and Actinobacteria, and negatively correlated to the relative abundance of Nitrospira and Acidobacteria. Together, our results support the copiotrophic/oligotrophic model of soil heterotrophic microbes suggested by Fierer et al. (2007). (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2153 / 2160
页数:8
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