Aminopeptidase N (APN) isoforms were identified as candidate receptors for Bacillus thuringiensis Cry toxins from the midgut of several insect species. In this study a partial cDNA encoding aminopeptidase (slfbAPN) was cloned from fat body of the moth Spodoptera litura. In the deduced amino acid sequence the characteristic metallopeptidase sequences, HEXXHX18E and GAMENWG were conserved but the sequence showed only 33-39% identity to other insect APNs, which were also reported to be Cry toxin receptors. The presence of a putative GPI anchor signal sequence at the C-terminus indicated that it is a membrane-anchored protein. The slfbAPN expression was restricted to the fat body as suggested by northern blot analysis of different tissues. Biochemical analyses including immunoblotting, ligand blotting and lectin blotting, demonstrated that slfbAPN is a membrane-anchored glycoprotein in the fat body and it binds to Cry toxins.
机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Li, Ming Shun
Choi, Jae Young
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Choi, Jae Young
Roh, Jong Yul
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Roh, Jong Yul
Shim, Hee Jin
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Shim, Hee Jin
Kang, Joong Nam
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Kang, Joong Nam
Kim, Yang-Su
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Kim, Yang-Su
Wang, Yong
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Wang, Yong
Yu, Zi Niu
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Yu, Zi Niu
Jin, Byung Rae
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机构:Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
Jin, Byung Rae
Je, Yeon Ho
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Seoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South KoreaSeoul Natl Univ, Sch Agr Biotechnol, Coll Agr & Life Sci, Seoul 151742, South Korea
机构:
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
JIN Ming-hui
TAO Jia-hui
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Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
TAO Jia-hui
LI Qi
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Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
LI Qi
CHENG Ying
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Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
CHENG Ying
SUN Xiao-xu
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State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
SUN Xiao-xu
WU Kong-ming
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State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
WU Kong-ming
XIAO Yu-tao
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Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural SciencesAgricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
机构:
Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Jin Ming-hui
Tao Jia-hui
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Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Tao Jia-hui
Li Q
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Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Li Q
Cheng Ying
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Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Cheng Ying
Sun Xiao-xu
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Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Sun Xiao-xu
Wu Kong-ming
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Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
Wu Kong-ming
Xiao Yu-tao
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Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R ChinaChinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China