Detection of plant virus particles with a capacitive field-effect sensor

被引：17

作者：

Jablonski, Melanie ^{[1
,2
]}

Poghossian, Arshak ^{[3
]}

Keusgen, Michael ^{[2
]}

Wege, Christina ^{[4
]}

Schoening, Michael J. ^{[1
,5
]}

机构：

[1] FH Aachen, Inst Nano & Biotechnol, Heinrich Mumann Str 1, D-52428 Julich, Germany

[2] Philipps Univ Marburg, Inst Pharmaceut Chem, Marbacher Weg 6-10, D-35032 Marburg, Germany

[3] MicroNanoBio, Liebigstr 4, D-40479 Dusseldorf, Germany

[4] Univ Stuttgart, Inst Biomat & Biomol Syst, Ffaffenwaldring 57, D-70569 Stuttgart, Germany

[5] Forschungszentrum Julich, Inst Biol Informat Proc IBI 3, D-52428 Julich, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2021年 / 413卷 / 22期

关键词：

Plant virus; Capacitive field-effect sensor; Tobacco mosaic virus (TMV); Label-free detection; Zeta potential; TOBACCO-MOSAIC-VIRUS; LOSSES; IMMOBILIZATION; BIOSENSORS; DISEASE; DEVICES; TOMATO; PEPPER; ASSAY; PH;

D O I：

10.1007/s00216-021-03448-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant viruses are major contributors to crop losses and induce high economic costs worldwide. For reliable, on-site and early detection of plant viral diseases, portable biosensors are of great interest. In this study, a field-effect SiO2-gate electrolyte-insulator-semiconductor (EIS) sensor was utilized for the label-free electrostatic detection of tobacco mosaic virus (TMV) particles as a model plant pathogen. The capacitive EIS sensor has been characterized regarding its TMV sensitivity by means of constant-capacitance method. The EIS sensor was able to detect biotinylated TMV particles from a solution with a TMV concentration as low as 0.025 nM. A good correlation between the registered EIS sensor signal and the density of adsorbed TMV particles assessed from scanning electron microscopy images of the SiO2-gate chip surface was observed. Additionally, the isoelectric point of the biotinylated TMV particles was determined via zeta potential measurements and the influence of ionic strength of the measurement solution on the TMV-modified EIS sensor signal has been studied.

引用

页码：5669 / 5678

页数：10

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