Does maternal-fetal transfer of creatine occur in pregnant sheep?

被引:17
作者
Baharom, Syed [1 ,2 ,5 ]
De Matteo, Robert [2 ]
Ellery, Stacey [1 ]
Della Gatta, Paul [3 ]
Bruce, Clinton R. [3 ]
Kowalski, Greg M. [3 ]
Hale, Nadia [1 ]
Dickinson, Hayley [1 ]
Harding, Richard [2 ]
Walker, David [1 ,4 ]
Snow, Rodney J. [3 ]
机构
[1] Hudson Inst Med Res, Ritchie Ctr, Clayton, Vic, Australia
[2] Monash Univ, Dept Anat & Dev Biol, Melbourne, Vic, Australia
[3] Deakin Univ, Inst Phys Act & Nutr, Melbourne, Vic, Australia
[4] Monash Univ, Dept Obstet & Gynaecol, Melbourne, Vic, Australia
[5] Univ Teknol MARA, Fac Med, Sungai Buloh, Selangor, Malaysia
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 2017年 / 313卷 / 01期
基金
英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词
creatine; guanidinoacetic acid; placenta; fetus; pregnancy; NUCLEAR-MAGNETIC-RESONANCE; HIGH-ENERGY PHOSPHATES; SPINY MOUSE; PLACENTAL TRANSPORT; AMNIOTIC-FLUID; IN-VIVO; RAT; GUANIDINOACETATE; METABOLISM; BRAIN;
D O I
10.1152/ajpendo.00450.2016
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Our aim was to determine the disposition of creatine in ovine pregnancy and whether creatine is transferred across the placenta from mother to fetus. Pregnant ewes received either 1) a continuous intravenous infusion of creatine monohydrate or saline from 122 to 131 days gestation, with maternal and fetal arterial blood and amniotic fluid samples collected daily for creatine analysis and fetal tissues collected at necropsy at 133 days for analysis of creatine content, or 2) a single systemic bolus injection of [C-13] creatine monohydrate at 130 days of gestation, with maternal and fetal arterial blood, uterine vein blood, and amniotic fluid samples collected before and for 4 h after injection and analyzed for creatine, creatine isotopic enrichment, and guanidinoacetic acid (GAA; precursor of creatine) concentrations. Presence of the creatine transporter-1 (SLC6A8) and L-arginine: glycine amidinotransferase (AGAT; the enzyme synthesizing GAA) proteins were determined by Western blots of placental cotyledons. The 10-day creatine infusion increased maternal plasma creatine concentration three-to fourfold (P < 0.05) without significantly changing fetal arterial, amniotic fluid, fetal tissues, or placental creatine content. Maternal arterial C-13 enrichment was increased (P < 0.05) after bolus [C-13] creatine injection without change of fetal arterial C-13 enrichment. SLC6A8 and AGAT proteins were identified in placental cotyledons, and GAA concentration was significantly higher in uterine vein than maternal artery plasma. Despite the presence of SLC6A8 protein in cotyledons, these results suggest that creatine is not transferred from mother to fetus in near-term sheep and that the ovine utero-placental unit releases GAA into the maternal circulation.
引用
收藏
页码:E75 / E83
页数:9
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