Roles of Endogenous IL-10 and IL-10-Competent and CD5+ B Cells in Autoimmune Thyroiditis in NOD.H-2h4 Mice

被引：4

作者：

Qin, Jing ^{[1
,3
]}

Zhao, Na ^{[1
]}

Wang, Shuo ^{[1
]}

Liu, Shanshan ^{[2
]}

Liu, Yongping ^{[1
]}

Cui, Xuejiao ^{[1
]}

Wang, Shiwei ^{[1
]}

Xiang, Yang ^{[1
]}

Fan, Chenling ^{[1
]}

Li, Yushu ^{[1
]}

Shan, Zhongyan ^{[1
]}

Teng, Weiping ^{[1
]}

机构：

[1] China Med Univ, Affiliated Hosp 1, Liaoning Prov Key Lab Endocrine Dis, Dept Endocrinol & Metab,Inst Endocrinol, 155 Nanjing North St, Shenyang 110001, Liaoning, Peoples R China

[2] 202 Hosp Peoples Liberat Army, Dept Emergency, Shenyang 110001, Liaoning, Peoples R China

[3] Inner Mongolia Med Univ, Affiliated Hosp, Dept Endocrinol, 1 Tongdao North St, Hohhot 010000, Inner Mongolia, Peoples R China

来源：

ENDOCRINOLOGY | 2020年 / 161卷 / 04期

关键词：

IL-10; CD1d; CD5; autoimmune thyroiditis; T-CELLS; IFN-GAMMA; TGF-BETA; EXPRESSION; MOUSE; IODIDE; SYMPORTER; DISEASES; CANCER;

D O I：

10.1210/endocr/bqaa033

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Interleukin (IL)-10 is a highly important anti-inflammatory cytokine in the immune system. CD1d(hi) and CD5(+) B cells are both traditionally defined IL-10-secreting B cells. In recent years, a B cell group with combined markers of CD1d(hi) and CD5(+) has been widely studied as it has been reported to suppress autoimmunity in mouse models of autoimmune diseases through IL-10 mechanisms. From the perspective of origination, CD1d(hi) and CD5(+) B cells are developed from different B cell lineages. Whether the regulatory capacity of these 2 B cell groups is consistent with their ability to secrete IL-10 has not been determined. In this study, we generated IL-10 knockout NOD.H-2(h4) mice to investigate the function of endogenous IL-10 in autoimmune thyroiditis and conducted adoptive transfer experiments to explore the respective roles of CD5(+) and CD1d(hi) B cells. In our results, the IL-10(-/-) NOD.H-2(h4) mice developed thyroiditis, similar to wild-type NOD.H-2(h4) mice. The CD5(+) B cells were more capable of secreting IL-10 than CD1d(hi) B cells in flow cytometric analysis, but the CD1d(hi) B cells showed more suppressive effects on thyroiditis development and autoantibody production, as well as Th17 cell response. In conclusion, endogenous IL-10 does not play an important role in autoimmune thyroiditis. CD1d(hi) B cells may play regulatory roles through mechanisms other than secreting IL-10.

引用

页数：11

共 48 条

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