Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes

被引：116

作者：

Dash, Rupesh ^{[1
]}

Mandal, Mahitosh ^{[2
]}

Ghosh, Sudip K. ^{[1
]}

Kundu, S. C. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

[2] Indian Inst Technol, Sch Med Sci & Technol, Kharagpur, W Bengal, India

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2008年 / 311卷 / 1-2期

关键词：

sericin; keratinocytes; UVB; H(2)O2;

D O I：

10.1007/s11010-008-9702-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The silk protein sericin has been identified as a potent antioxidant and photoprotective agent against ultraviolet B (UVB) irradiation in mouse skin model. In this study, we have investigated the anti-apoptotic effect of sericin in UVB (30 mJ/cm(2))-irradiated human keratinocytes. Flow cytometry analysis has shown that pre-treatment with sericin inhibits UVB-induced apoptosis. The pre-treatment with sericin suppresses bax expression, up-regulates the expression of bcl-2, prevents both the activation of caspase-3 and cleavage of Poly (ADP-ribose) polymerase. Generation of intracellular hydrogen peroxide in UVB-treated keratinocytes is inhibited through pre-treatment with sericin suggesting that sericin probably prevents mitochondrial damage.

引用

页码：111 / 119

页数：9

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