Evaluation of nutritional and genetic determinants of total homocysteine, methylmalonic acid and S-adenosylmethionine/S-adenosylhomocysteine values in Brazilian childbearing-age women

被引：15
作者：
Barbosa, Patricia R. [1 ]
Stabler, Sally P. [2 ]
Trentin, Renata [1 ]
Carvalho, Felipe R. [1 ]
Luchessi, Andre D. [1 ]
Hirata, Rosario D. C. [1 ]
Hirata, Mario H. [1 ]
Allen, Robert H. [2 ]
Guerra-Shinohara, Elvira M. [1 ]
机构：
[1] Univ Sao Paulo, Dept Clin Chem & Toxicol, Sch Pharmaceut Sci, BR-05508900 Sao Paulo, Brazil
[2] Univ Colorado, Hlth Sci Ctr, Dept Med, Denver, CO 80262 USA
来源：
CLINICA CHIMICA ACTA | 2008年 / 388卷 / 1-2期
关键词：
cobalamin; folate; polymorphisms; homocysteine; methylmalonic acid; women; METHIONINE SYNTHASE REDUCTASE; REDUCED FOLATE CARRIER; GLUTAMATE CARBOXYPEPTIDASE-II; PLASMA HOMOCYSTEINE; METHYLENETETRAHYDROFOLATE REDUCTASE; TRANSCOBALAMIN GENE; D919G POLYMORPHISM; PREGNANT-WOMEN; RISK-FACTOR; COBALAMIN;
D O I：
10.1016/j.cca.2007.10.023
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Cobalamin (Cbl) and folate deficiencies and gene polymorphism of key enzymes or carriers can impair homocysteine metabolism and may change the serum values of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). We investigated the nutritional and genetic determinants for total homocysteine (tHcy), methylmalonic acid (MMA) and SAM/SAH in healthy Brazilian childbearing-age women. Methods: Serum concentrations of Cbl, folate, red blood cell folate, ferritin, tHcy, MMA, SAM, SAH and other metabolites were measured in 102 healthy unrelated women. The genotypes for MTHFR C677T, MTHFR A1298C, MTR A2756G, MTRR A66G, TC2 C776G, TC2 A67G and RFCI A80G gene polymorphisms were identified by PCR-RFLP. Results: Serum folate and Cbl were inversely correlated with tHcy and serum MMA, respectively. Cbl deficiency was associated with increased MMA and reduced alpha-aminobutyrate, serine and N-methylglycine concentrations. No variable was associated with SAM/SAH ratio. In addition, gene polymorphisms were not selected as determinants for tHcy, MMA and SAM/SAH ratio. Iron, Cbl and folate deficiencies were found respectively in 30.4%, 22.5% and 2.0% of individuals studied. Conclusions: There was a high frequency of Cbl and iron deficiency in this group of childbearing-age women. Serum folate and Cbl were the determinants of serum tHcy and MMA concentration, respectively. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：139 / 147
页数：9
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