Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells

被引：8

作者：

Ferguson, Elaine L. ^{[1
,2
]}

Naseer, Sameza ^{[1
]}

Powell, Lydia C. ^{[1
,2
]}

Hardwicke, Joseph ^{[1
,2
,3
,4
]}

Young, Fraser, I ^{[1
,2
]}

Zhu, Bangfu ^{[1
,5
]}

Liu, Qian ^{[1
,6
]}

Song, Bing ^{[1
,2
]}

Thomas, David W. ^{[1
,2
]}

机构：

[1] Cardiff Univ, Coll Biomed & Life Sci, Sch Dent, Oral & Biomed Sci, Heath Pk, Cardiff CF14 4XY, S Glam, Wales

[2] Cardiff Univ, Cardiff Inst Tissue Engn & Repair, 10 Museum Pl, Cardiff CF10 3BG, S Glam, Wales

[3] Univ Hosp Coventry, Clifford Bridge Rd, Coventry CV2 2DX, W Midlands, England

[4] Univ Hosp Warwickshire, Clifford Bridge Rd, Coventry CV2 2DX, W Midlands, England

[5] Univ Bristol, Biomed Sci Bldg,Univ Walk, Clifton BS8 1TD, Avon, England

[6] Nanjing Univ, Jinling Hosp, Dept Neurol, Sch Med, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China

来源：

STEM CELL RESEARCH | 2018年 / 33卷

基金：

欧洲研究理事会;

关键词：

Polymer therapeutics; Biodegradable polymers; Growth factors; Neural stem cells; Controlled release; SPINAL-CORD; FUNCTIONAL RECOVERY; POLYMER THERAPEUTICS; SUSTAINED-RELEASE; DELIVERY; BRAIN; PROLIFERATION; NEURONS; RHEGF; OLIG2;

D O I：

10.1016/j.scr.2018.10.008

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (similar to 74,000 g/mol; 27.8 mol% succinoylation) conjugated to epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF; or FGF-2) (3.9 and 6.7% w/w protein loading, respectively) to support the expansion and differentiation of stem cells in vitro via sustained, controllable growth factor release. Supplementation of mouse neural stem cells (mNSCs) with dextrin-growth factor conjugates led to greater and prolonged proliferation compared to unbound EGF/bFGF controls, with no detectable apoptosis after 7 days of treatment. Immunocytochemical detection of neural precursor (nestin) and differentiation (Olig2, MAP2, GFAP) markers verified that controlled release of dextrin-conjugated growth factors preserves stem cell properties of mNSCs for up to 7 days. These results show the potential of dextrin-growth factor conjugates for localized delivery of bioactive therapeutic agents to support stem cell expansion and differentiation, and as an adjunct to direct neuronal repair.

引用

页码：69 / 78

页数：10

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