In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

被引：5

作者：

Brezna, Barbara ^{[1
]}

Smid, Jiri ^{[1
,4
]}

Costa, Joana ^{[2
]}

Radvanszky, Jan ^{[3
]}

Mafra, Isabel ^{[2
]}

Kuchta, Tomas ^{[1
]}

机构：

[1] Food Res Inst, Dept Microbiol & Mol Biol, Bratislava 82475, Slovakia

[2] Univ Porto, Fac Farm, Dept Ciencias Quim, REQUIMTE, P-4050313 Oporto, Portugal

[3] Slovak Acad Sci, Inst Mol Physiol & Genet, Bratislava 83334, Slovakia

[4] Brno Univ Technol, Fac Chem, Inst Food Chem & Biotechnol, Brno 61200, Czech Republic

来源：

MOLECULAR AND CELLULAR PROBES | 2015年 / 29卷 / 02期

关键词：

Almond; DNA; Real-time PCR; Food; Allergen; REAL-TIME PCR; MELTING ANALYSIS; ALLERGENS; MISMATCHES; GENES; QUANTIFICATION; IDENTIFICATION; POLYMORPHISMS; PRIMER; ASSAYS;

D O I：

10.1016/j.mcp.2014.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten published DNA-based analytical methods aiming at detecting material of almond (Prunus dulcis) were in silica evaluated for potential cross-reactivity with other stone fruits (Prunus spp.), including peach, apricot, plum, cherry, sour cherry and Sargent cherry. For most assays, the analysis of nucleotide databases suggested none or insufficient discrimination of at least some stone fruits. On the other hand, the assay targeting non-specific lipid transfer protein (Roder et al., 2011, Anal Chim Acta 685:74-83) was sufficiently discriminative, judging from nucleotide alignments. Empirical evaluation was performed for three of the published methods, one modification of a commercial kit (SureFood allergen almond) and one attempted novel method targeting thaumatin-like protein gene. Samples of leaves and kernels were used in the experiments. The empirical results were favourable for the method from Roder et al. (2011) and a modification of SureFood allergen almond kit, both showing cross-reactivity <10(-3) compared to the model almond. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：99 / 115

页数：17