Molecular cloning of canine Wilms' tumor 1 for immunohistochemical analysis in canine tissues

被引：4

作者：

Sakai, Osamu ^{[1
]}

Sakurai, Masashi ^{[2
]}

Sakai, Hiroki ^{[3
]}

Kubo, Masahito ^{[2
]}

Hiraoka, Hiroko ^{[1
]}

Baba, Kenji ^{[4
]}

Okuda, Masaru ^{[4
]}

Mizuno, Takuya ^{[1
]}

机构：

[1] Yamaguchi Univ, United Grad Sch Vet Med, Lab Mol Diagnost & Therapeut, 1677-1 Yoshida, Yamaguchi 7538515, Japan

[2] Yamaguchi Univ, Joint Fac Vet Med, Lab Vet Pathol, Yamaguchi 7538515, Japan

[3] Gifu Univ, Lab Vet Pathol, Pathogenet Vet Med, Joint Dept Vet Med,Fac Appl Biol Sci, Gifu 5011193, Japan

[4] Yamaguchi Univ, Joint Fac Vet Med, Lab Vet Internal Med, Yamaguchi 7538515, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2017年 / 79卷 / 07期

关键词：

cancer; canine; immunohistochemical staining; Wilms' tumor 1; WT1 PEPTIDE VACCINE; GENE-PRODUCT; CANCER; PATIENT; CML;

D O I：

10.1292/jvms.17-0229

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Wilms' tumor 1 (WT1) expression has been investigated in various human cancers as a target molecule for cancer immunotherapy. However, few studies have focused on WT1 expression in dogs. Firstly, cDNA of canine WT1 (cWT1) was molecularly cloned from normal canine kidney. The cross-reactivity of the anti-human WT1 monoclonal antibody (6F-H2) with cWT1 was confirmed via Western blotting using cells overexpressing cWT1. Immunohistochemical staining revealed that cWT1 expression was detected in all canine lymphoma tissues and in some normal canine tissues, including the kidney and lymph node. cWT1 is a potential immunotherapy target against canine cancers.

引用

页码：1272 / 1277

页数：6

共 50 条

[21] Molecular cloning of canine thymus and activation-regulated chemokine (TARC) gene and its expression in various tissues
Maeda, S
Mizuno, T
Yamashita, K
Kurata, K
Masuda, K
Ohno, K
Tsujimoto, H
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2001, 63 (09) : 1035 - 1038
[22] Cloning of canine myocilin cDNA and molecular analysis of the myocilin gene in Shiba Inu dogs
Kato, Kumiko
Sasaki, Nobuo
Matsunaga, Satoru
Nishimura, Ryohei
Ogawa, Hiroyuki
VETERINARY OPHTHALMOLOGY, 2007, 10 : 53 - 62
[23] Molecular cloning of canine activation-induced cytidine deaminase (AID) cDNA and its expression in normal tissues
Ohmori, K
Maeda, S
Okayama, T
Masuda, K
Ohno, K
Tsujimoto, H
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2004, 66 (06) : 739 - 741
[24] Detection of indoleamine 2,3-dioxygenase 1-expressing cells in canine normal and tumor tissues
Ikeda, Namiko
Kato, Daiki
Tsuboi, Masaya
Yoshitake, Ryohei
Eto, Shotaro
Yoshimoto, Sho
Shinada, Masahiro
Kamoto, Satoshi
Hashimoto, Yuko
Takahashi, Yousuke
Chambers, James
Uchida, Kazuyuki
Nishimura, Ryohei
Nakagawa, Takayuki
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2021, 83 (12) : 1885 - 1890
[25] Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage
Tomura, Shintaro
Uchida, Mona
Yonezawa, Tomohiro
Kobayashi, Masato
Bonkobara, Makoto
Arai, Satoko
Miyazaki, Toru
Tamahara, Satoshi
Matsuki, Naoaki
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2014, 76 (12) : 1641 - 1645
[26] Immunohistochemical expression of Epidermal Growth Factor Receptor (EGFR) in canine mammary tissues
Gama, A.
Gaertner, F.
Alves, A.
Schmitt, F.
RESEARCH IN VETERINARY SCIENCE, 2009, 87 (03) : 432 - 437
[27] Canine transmissible venereal tumor - From general to molecular characteristics: A review
Faro, Thamirys A. S.
de Oliveira, Edivaldo H. C.
ANIMAL GENETICS, 2023, 54 (01) : 82 - 89
[28] The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors
Otsuka, Narumi
Ishimaru, Kairi
Murakami, Mami
Goto, Minami
Hirata, Akihiro
Sakai, Hiroki
JOURNAL OF VETERINARY MEDICAL SCIENCE, 2022, 84 (07) : 914 - 923
[29] Molecular cloning and sequence analysis of canine acidic ribosomal phosphoprotein P0 cDNA
Canatan, H
Lin, YC
DNA SEQUENCE, 2000, 11 (3-4): : 321 - 326
[30] MOLECULAR-CLONING AND EXPRESSION OF CANINE INTERLEUKIN-8 CDNA
MATSUMOTO, Y
MOHAMED, A
ONODERA, T
KATO, H
OHASHI, T
GOITSUKA, R
TSUJIMOTO, H
HASEGAWA, A
FURUSAWA, S
YOSHIHARA, K
ISHIKAWA, J
HOTTA, K
SUZUKI, K
HIROTA, Y
CYTOKINE, 1994, 6 (05) : 455 - 461

← 1 2 3 4 5 →