Role of mRNA stability in the different patterns of cytokine production by CD4+ cells from young and old mice

被引:27
|
作者
Pioli, C
Pucci, S
Barile, S
Frasca, D
Doria, G
机构
[1] ENEA CR Casaccia, Immunol Lab, AMB PRO TOSS, I-00060 Rome, Italy
[2] Univ Rome, Dept Biol, Rome, Italy
关键词
D O I
10.1046/j.1365-2567.1998.00523.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD4(+) cells from young (3 months) and old (19 months) mice were stimulated by plate-bound anti-CD3 monoclonal antibody (mAb) alone or also by soluble anti-CD28 mAb. Supernatants were analysed by enzyme-linked immunosorbent assay (ELISA) to determine cytokine concentrations. Total RNA was extracted from cells, reverse transcribed and the cDNA amplified by polymerase chain reaction (PCR) to evaluate the amount of specific mRNA. The results indicate that anti-CD3 alone is not sufficient to induce interleukin-2 (IL-2) production in CD4(+) cells from both young and old mice. However, anti-CD28, together with anti-CD3 mAb, induces a much higher production of IL-2 in CD4(+) cells from young as compared with old mice. Conversely, interferon-gamma (IFN-gamma) production is also induced by anti-CD3 alone and is higher in CD4(+) cells from old as compared with young mice. Upon addition of anti-CD28 mAb, IFN-gamma production increases in both groups, but it remains much higher in old than in young mice. Also the production of IL-4 and IL-10 is induced by anti-CD3 mAb but it is increased by the addition of anti-CD28 mAb. CD4(+) cells from old mice produce more IL-4 and IL-10 as compared with cells from young mice. The amounts of cytokine specific mRNA in CD4(+) cells from young and old mice parallel the cytokine levels in culture supernatants. Results on the mRNA turnover indicate that when CD4(+) cells are stimulated by anti-CD3 or costimulated also by anti-CD28 mAb, the IFN-gamma, IL-4 and IL-10 specific mRNAs are more stable in old than in young mice, suggesting that mRNA stability has a relevant role in the different patterns of cytokine production.
引用
收藏
页码:380 / 387
页数:8
相关论文
共 50 条
  • [1] PATTERNS OF CYTOKINE GENE-EXPRESSION BY CD4+ T-CELLS FROM YOUNG AND OLD MICE
    HOBBS, MV
    WEIGLE, WO
    NOONAN, DJ
    TORBETT, BE
    MCEVILLY, RJ
    KOCH, RJ
    CARDENAS, GJ
    ERNST, DN
    JOURNAL OF IMMUNOLOGY, 1993, 150 (08): : 3602 - 3614
  • [2] CELL-PROLIFERATION AND CYTOKINE PRODUCTION BY CD4+ CELLS FROM OLD MICE
    HOBBS, MV
    ERNST, DN
    TORBETT, BE
    GLASEBROOK, AL
    REHSE, MA
    MCQUITTY, DN
    THOMAN, ML
    BOTTOMLY, K
    ROTHERMEL, AL
    NOONAN, DJ
    WEIGLE, WO
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 46 (04) : 312 - 320
  • [3] Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells
    Zhang, Xiansong
    Li, Shuai
    Luo, Yu
    Chen, Yingyu
    Cheng, Shi
    Zhang, Guangzhi
    Hu, Changmin
    Chen, Huanchun
    Guo, Aizhen
    MICROBIOLOGY-SGM, 2013, 159 : 366 - 379
  • [4] A glycoprotein endopeptidase enhances calcium influx and cytokine production by CD4+T cells of old and young mice
    Berger, SB
    Akha, AAS
    Miller, RA
    INTERNATIONAL IMMUNOLOGY, 2005, 17 (08) : 983 - 991
  • [5] A glycoprotein endopeptidase enhances calcium influx and cytokine production by CD4+T cells of old and young mice
    Berger, SB
    Akha, AAS
    Miller, RA
    FASEB JOURNAL, 2005, 19 (04): : A958 - A958
  • [6] REDUCTION OF CD4+ T CELLS NEGATIVELY AFFECTS SURVIVAL IN OLD BUT NOT YOUNG MICE WITH GLIOBLASTOMA
    Zhai, Lijie
    Bell, April
    Lauing, Kristen
    Bommi, Prashant
    Ladomersky, Erik
    Mi, Xinlei
    Kocherginski, Masha
    Chandler, James
    Horbinski, Craig
    Heimberger, Amy
    Lukas, Rimas
    Wainwright, Derek
    NEURO-ONCOLOGY, 2022, 24 : 132 - 132
  • [7] Regulation of cytokine production by CD4+ and CD8+ T cells
    Corbin, GA
    Harty, JT
    FASEB JOURNAL, 2004, 18 (04): : A436 - A437
  • [8] A glycoprotein endopeptidase that enhances cytokine production by CD4+T cells of old mice
    Berer, S
    Miller, R
    GERONTOLOGIST, 2004, 44 : 26 - 26
  • [9] Cytokine production by CD4+ T cells of mice immunised with antigen-liposome conjugates.
    Horino, A
    Naito, S
    Taneichi, M
    Kato, H
    Komuro, K
    Uchida, T
    JOURNAL OF LEUKOCYTE BIOLOGY, 1999, : 18 - 18
  • [10] MATURATIONAL CHANGES IN CD4+ CELL SUBSETS AND CYTOKINE PRODUCTION IN BXSB MICE
    CHU, EB
    WEIGLE, WO
    JOURNAL OF IMMUNOLOGY, 1993, 150 (08): : A31 - A31