Enhancement of Targeted Homologous Recombination in Mycoplasma mycoides subsp. capri by Inclusion of Heterologous recA

被引：18

作者：

Allam, Ayman B. ^{[1
]}

Reyes, Leticia ^{[1
]}

Assad-Garcia, Nacyra ^{[2
]}

Glass, John I. ^{[2
]}

Brown, Mary B. ^{[1
]}

机构：

[1] Univ Florida, Coll Vet Med, Dept Infect Dis & Pathol, Gainesville, FL 32611 USA

[2] J Craig Venter Inst, Rockville, MD USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 20期

关键词：

TRANSPOSON MUTAGENESIS; ACHOLEPLASMA-LAIDLAWII; VIRULENCE DETERMINANT; TERMINAL ORGANELLE; SPIROPLASMA-CITRI; ORIC PLASMIDS; PULMONIS; GENOME; CONSTRUCTION; REPLICATION;

D O I：

10.1128/AEM.00056-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A suicide plasmid, pExp1-ctpA::tetM-recAec, employing recA from Escherichia coli and tetM as a selection marker, was used to generate ctpA knockout mutants in Mycoplasma mycoides subsp. capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both the consistency and the recovery of mutants generated by homologous recombination.

引用

页码：6951 / 6954

页数：4

共 3 条

[1] E-coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis
Ishag, Hassan Z. A.
Xiong, Qiyan
Liu, Maojun
Feng, Zhixin
Shao, Guoqing
JOURNAL OF MICROBIOLOGICAL METHODS, 2017, 136 : 49 - 56
[2] CLONING AND CHARACTERIZATION OF THE RECA GENES FROM MYCOPLASMA-PULMONIS AND M-MYCOIDES SUBSP MYCOIDES
KING, KW
WOODARD, A
DYBVIG, K
GENE, 1994, 139 (01) : 111 - 115
[3] High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237
Anne Fischer
Ivette Santana-Cruz
Jan Hegerman
Hadrien Gourlé
Elise Schieck
Mathieu Lambert
Suvarna Nadendla
Hezron Wesonga
Rachel A. Miller
Sanjay Vashee
Johann Weber
Jochen Meens
Joachim Frey
Joerg Jores
Standards in Genomic Sciences, 10

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