Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood

被引：25

作者：

Matsumoto, Akihiro ^{[1
]}

Takahashi, Yuki ^{[1
]}

Ogata, Kosuke ^{[2
]}

Kitamura, Shimpei ^{[1
]}

Nakagawa, Naoki ^{[1
]}

Yamamoto, Aki ^{[1
]}

Ishihama, Yasushi ^{[2
]}

Takakura, Yoshinobu ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Biopharmaceut & Drug Metab, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Mol & Cellular BioAnal, Kyoto 6068501, Japan

来源：

ISCIENCE | 2021年 / 24卷 / 08期

基金：

日本学术振兴会;

关键词：

EXOSOMES; DELIVERY; LIPIDOMICS; PHENOTYPE; PLATFORM;

D O I：

10.1016/j.isci.2021.102839

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Small extracellular vesicles (sEVs) are important mediators of intercellular communication with respect to diverse pathophysiological processes. Here, we determined novel phosphatidylserine (PS)-deficient sEV subpopulations as a major somatic cell-derived sEV subpopulation in blood because of long blood circulation half-life through escape from macrophage uptake. PS(-)-sEVs were identified in various cultured cells as aminor population. However, as a result of rapid uptake of PS(+)-sEVs by macrophages, circulating somatic cell-derived sEVs in the blood were found to be mainly PS(-)-sEVs. These results suggest that endogenous PS(-)-sEVs could indeed be the key player in sEV-mediated intercellular communication, a good target for sEV-based diagnosis, and a potent candidate for sEV-based drug delivery. Our findings bring a paradigm shift in the understanding of the biology and translational applications of sEVs.

引用

页数：19

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