Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood

被引：25

作者：

Matsumoto, Akihiro ^{[1
]}

Takahashi, Yuki ^{[1
]}

Ogata, Kosuke ^{[2
]}

Kitamura, Shimpei ^{[1
]}

Nakagawa, Naoki ^{[1
]}

Yamamoto, Aki ^{[1
]}

Ishihama, Yasushi ^{[2
]}

Takakura, Yoshinobu ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Biopharmaceut & Drug Metab, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Mol & Cellular BioAnal, Kyoto 6068501, Japan

来源：

ISCIENCE | 2021年 / 24卷 / 08期

基金：

日本学术振兴会;

关键词：

EXOSOMES; DELIVERY; LIPIDOMICS; PHENOTYPE; PLATFORM;

D O I：

10.1016/j.isci.2021.102839

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Small extracellular vesicles (sEVs) are important mediators of intercellular communication with respect to diverse pathophysiological processes. Here, we determined novel phosphatidylserine (PS)-deficient sEV subpopulations as a major somatic cell-derived sEV subpopulation in blood because of long blood circulation half-life through escape from macrophage uptake. PS(-)-sEVs were identified in various cultured cells as aminor population. However, as a result of rapid uptake of PS(+)-sEVs by macrophages, circulating somatic cell-derived sEVs in the blood were found to be mainly PS(-)-sEVs. These results suggest that endogenous PS(-)-sEVs could indeed be the key player in sEV-mediated intercellular communication, a good target for sEV-based diagnosis, and a potent candidate for sEV-based drug delivery. Our findings bring a paradigm shift in the understanding of the biology and translational applications of sEVs.

引用

页数：19

共 33 条

[1] Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes [J].

Alvarez-Erviti, Lydia ;

Seow, Yiqi ;

Yin, HaiFang ;

Betts, Corinne ;

Lakhal, Samira ;

Wood, Matthew J. A. .

NATURE BIOTECHNOLOGY, 2011, 29 (04) :341-U179

[2] Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration [J].

Arraud, N. ;

Linares, R. ;

Tan, S. ;

Gounou, C. ;

Pasquet, J. -M. ;

Mornet, S. ;

Brisson, A. R. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2014, 12 (05) :614-627

[3] Cell type-specific and common characteristics of exosomes derived from mouse cell lines: Yield, physicochemical properties, and pharmacokinetics [J].

Charoenviriyakul, Chonlada ;

Takahashi, Yuki ;

Morishita, Masaki ;

Matsumoto, Akihiro ;

Nishikawa, Makiya ;

Takakura, Yoshinobu .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2017, 96 :316-322

[4] Role of Extracellular Vesicle Surface Proteins in the Pharmacokinetics of Extracellular Vesicles [J].

Charoenyiriyakul, Chonlada ;

Takahashi, Yuki ;

Morishita, Masaki ;

Nishikawa, Makiya ;

Takakura, Yoshinobu .

MOLECULAR PHARMACEUTICS, 2018, 15 (03) :1073-1080

[5] Circulating exosomes carrying an immunosuppressive cargo interfere with cellular immunotherapy in acute myeloid leukemia [J].

Hong, Chang-Sook ;

Sharma, Priyanka ;

Yerneni, Saigopalakrishna S. ;

Simms, Patricia ;

Jackson, Edwin K. ;

Whiteside, Theresa L. ;

Boyiadzis, Michael .

SCIENTIFIC REPORTS, 2017, 7

[6] Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer [J].

Hong, Chang-Sook ;

Funk, Sonja ;

Muller, Laurent ;

Boyiadzis, Michael ;

Whiteside, Theresa L. .

JOURNAL OF EXTRACELLULAR VESICLES, 2016, 5

[7] Tumour exosome integrins determine organotropic metastasis [J].

Hoshino, Ayuko ;

Costa-Silva, Bruno ;

Shen, Tang-Long ;

Rodrigues, Goncalo ;

Hashimoto, Ayako ;

Mark, Milica Tesic ;

Molina, Henrik ;

Kohsaka, Shinji ;

Di Giannatale, Angela ;

Ceder, Sophia ;

Singh, Swarnima ;

Williams, Caitlin ;

Soplop, Nadine ;

Uryu, Kunihiro ;

Pharmer, Lindsay ;

King, Tari ;

Bojmar, Linda ;

Davies, Alexander E. ;

Ararso, Yonathan ;

Zhang, Tuo ;

Zhang, Haiying ;

Hernandez, Jonathan ;

Weiss, Joshua M. ;

Dumont-Cole, Vanessa D. ;

Kramer, Kimberly ;

Wexler, Leonard H. ;

Narendran, Aru ;

Schwartz, Gary K. ;

Healey, John H. ;

Sandstrom, Per ;

Labori, Knut Jorgen ;

Kure, Elin H. ;

Grandgenett, Paul M. ;

Hollingsworth, Michael A. ;

de Sousa, Maria ;

Kaur, Sukhwinder ;

Jain, Maneesh ;

Mallya, Kavita ;

Batra, Surinder K. ;

Jarnagin, William R. ;

Brady, Mary S. ;

Fodstad, Oystein ;

Muller, Volkmar ;

Pantel, Klaus ;

Minn, Andy J. ;

Bissell, Mina J. ;

Garcia, Benjamin A. ;

Kang, Yibin ;

Rajasekhar, Vinagolu K. ;

Ghajar, Cyrus M. .

NATURE, 2015, 527 (7578) :329-+

[8] Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer [J].

Kamerkar, Sushrut ;

LeBleu, Valerie S. ;

Sugimoto, Hikaru ;

Yang, Sujuan ;

Ruivo, Carolina F. ;

Melo, Sonia A. ;

Lee, J. Jack ;

Kalluri, Raghu .

NATURE, 2017, 546 (7659) :498-+

[9] Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins [J].

Karimi, Nasibeh ;

Cvjetkovic, Aleksander ;

Jang, Su Chul ;

Crescitelli, Rossella ;

Feizi, Mohammad Ali Hosseinpour ;

Nieuwland, Rienk ;

Lotvall, Jan ;

Lasser, Cecilia .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2018, 75 (15) :2873-2886

[10] Engineering macrophage-derived exosomes for targeted paclitaxel delivery to pulmonary metastases: in vitro and in vivo evaluations [J].

Kim, Myung Soo ;

Haney, Matthew J. ;

Zhao, Yuling ;

Yuan, Dongfen ;

Deygen, Irina ;

Klyachko, Natalia L. ;

Kabanov, Alexander V. ;

Batrakova, Elena V. .

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2018, 14 (01) :195-204

← 1 2 3 4 →