Gene for A-type inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses

被引：104

作者：

Meyer, H ^{[1
]}

Ropp, SL ^{[1
]}

Esposito, JJ ^{[1
]}

机构：

[1] US PHS,CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIAL DIS,ATLANTA,GA 30333

来源：

JOURNAL OF VIROLOGICAL METHODS | 1997年 / 64卷 / 02期

关键词：

orthopoxvirus; PCR; differentiation; ATI-gene;

D O I：

10.1016/S0166-0934(96)02155-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Orthopoxvirus species were identified and differentiated by polymerase chain reaction amplification of genome DNA using a single primer-pair based on sequences coding for the major protein component of the cowpox virus acidophilic-type inclusion body (ATI). DNA available for 6 of 8 Old World (cowpox, variola, monkeypox, camelpox, ectromelia and vaccinia viruses) and 3 New World (skunkpox, volepox, and raccoonpox) resulted in amplicons that ranged in size from 510 to 1673 base pairs depending on the species, except for raccoonpox virus DNA which did not amplify. XbaI digest gel electrophoresis profiles of the amplicons improved resolution of the differences. (C) 1997 Elsevier Science B.V.

引用

页码：217 / 221

页数：5

共 11 条

[1] ORTHOPOXVIRUS DNA - A COMPARISON OF RESTRICTION PROFILES AND MAPS [J].