Impact of RGD Nanopatterns Grafted onto Titanium on Osteoblastic Cell Adhesion

被引:32
作者
Minh Ngoc Nguyen [1 ]
Lebarbe, Thomas [1 ]
Zouani, Omar F. [2 ]
Pichavant, Loic [1 ,2 ]
Durrieu, Marie-Christine [2 ]
Heroguez, Valerie [1 ]
机构
[1] Univ Bordeaux 1, Lab Chim Polymeres Organ, IPB ENSCBP, CNRS UMR5629, F-33607 Pessac, France
[2] Univ Bordeaux 1, IECB, CNRS UMR CBMN 5248, F-33607 Pessac, France
关键词
IN-VITRO; PEPTIDES; DIFFERENTIATION; HYDROGELS; IMPLANTS; BEHAVIOR; GLYCOL); WELL;
D O I
10.1021/bm201812u
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This work reports on the synthesis of titanium bone implants functionalized with nanoparticles (NPs) containing Arg-Gly-Asp-Cys peptide (RGDC) and shows the adhesion behavior of cells seeded on these materials. RGDC peptides were first: conjugated to a norbornenyl-poly(ethylene oxide) macromonomer (Nb-PEO). Then, functional NPs with a size of similar to 300 nm and constituted of polynorbornene core surrounded by poly(ethylene oxide) shell were prepared by ring-opening metathesis polymerization in dispersed medium. The grafting density of these NPs on the titanium surface is up to 2 NPs.mu m(-2) (80 pmol of RGDC per cm(-2) of NP surface). Cell adhesion was evaluated using preosteoblast cells (MC3T3-E1). Results of cells cultured for 24 h showed that materials grafted with NPs functionalized with RGDC peptides enhance specific cell adhesion and can create filopodia-like among NP sites by stressing the cells.
引用
收藏
页码:896 / 904
页数:9
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