Fate mapping of neural crest cells during eye development using a protein 0 promoter-driven transgenic technique

被引：8

作者：

Iwao, Keiichiro ^{[1
,2
]}

Inatani, Masaru ^{[1
]}

Okinami, Satoshi ^{[2
]}

Tanihara, Hidenobu ^{[1
]}

机构：

[1] Kumamoto Univ, Grad Sch Med Sci, Dept Ophthalmol & Visual Sci, Kumamoto 8608556, Japan

[2] Saga Univ, Fac Med, Dept Ophthalmol, Saga 840, Japan

来源：

GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY | 2008年 / 246卷 / 08期

关键词：

Cre recombinase; Rosa26 Cre reporter; anterior eye segment; Cre-loxP system; transgenic mice;

D O I：

10.1007/s00417-008-0845-0

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose To map neural crest cell fate during eye development. Methods Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination. Results beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle. Conclusions Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.

引用

页码：1117 / 1122

页数：6

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