Chiral separation of simendan enantiomers by capillary zone electrophoresis

The enantiomers of simendan was separated by capillary zone electrophoresis using beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration, methanol content and separation voltage were investigated. A baseline separation of simendan enantiomers was achieved with background electrolyte of 20 mmol/L borate, buffer ( pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50, V/V). The linear range for simcndan enantiomer was 25 similar to 500 mg/L (0.997). The RSDs of migration time and peak area were 1.9% and 4.0%, respectively. This method can be applied in qualitative and quantitative analysis for simendan enantiomers.