Stimulatory effect of laser irradiation on calcified nodule formation in human dental pulp fibroblasts

被引：19

作者：

Ohbayashi, E

Matsushima, K

Hosoya, S

Abiko, Y

Yamazaki, M

机构：

[1] Nihon Univ, Sch Dent, Dept Endodont, Matsudo, Chiba 271, Japan

[2] Nihon Univ, Sch Dent, Dept Biochem, Matsudo, Chiba 271, Japan

来源：

JOURNAL OF ENDODONTICS | 1999年 / 25卷 / 01期

关键词：

D O I：

10.1016/S0099-2399(99)80338-0

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The purpose of the present study was to investigate the effect of laser irradiation on calcified nodule formation in human dental pulp (HDP) cells. HDP cells were irradiated once with a Ga-Al-As laser for 5 and 10 min, and calcified nodule formation was determined by von Kossa staining. The laser irradiation increased the number of calcified nodules in a time-dependent manner. The activity of alkaline phosphatase and production of collagen and osteocalcin in conditioned medium were measured. Both were higher in the irradiated group than in the nonirradiated group. These results suggested that formation of calcified nodules in HDP cells, as well as in alkaline phosphatase activity, the production of collagen and osteocalcin were enhanced by laser irradiation.

引用

页码：30 / 33

页数：4

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