Genotypic Analysis of Enterococci Isolated from Fecal-Polluted Water from Different Sources by Pulsed-Field Gel Electrophoresis (PFGE) for Application to Microbial Source Tracking

被引：4

作者：

Furukawa, Takashi ^{[2
]}

Takahashi, Hironori ^{[1
]}

Yoshida, Terutoyo ^{[3
]}

Suzuki, Yoshihiro ^{[1
]}

机构：

[1] Miyazaki Univ, Fac Engn, Dept Civil & Environm Engn, Miyazaki 8892192, Japan

[2] Miyazaki Univ, Grad Sch Agr & Engn, Miyazaki 8892192, Japan

[3] Miyazaki Univ, Dept Biol Prod & Environm Sci, Fac Agr, Miyazaki 8892192, Japan

来源：

MICROBES AND ENVIRONMENTS | 2011年 / 26卷 / 02期

关键词：

pulsed-field gel electrophoresis (PFGE); genotypic analysis; enterococci; fecal-polluted water; microbial source tracking; ESCHERICHIA-COLI; PCR; IDENTIFICATION; RESISTANCE; QUALITY;

D O I：

10.1264/jsme2.ME10174

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

PFGE has potential applications in the source tracking of fecal pollution in aquatic environments. We tried to distinguish the genotypes of Enterococcus faecium collected from fecal-contaminated water using PFGE. Well identified 115 strains of E. faecium were classified into 25 PFGE patterns, and characteristics distinctive to each genotype were recognized. Analysis of the characteristics of genotypes using PFGE can be used to track source of fecal pollution.

引用

页码：181 / 183

页数：3

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