Proteomics analysis after traumatic brain injury in rats: the search for potential biomarkers

被引：9

作者：

Ding, Jun ^{[1
]}

Ding, Zhengang ^{[2
]}

Yuan, Fang ^{[1
]}

Guo, Jianyi ^{[1
]}

Chen, Hao ^{[1
]}

Gao, Wenwei ^{[1
]}

Wang, Ren ^{[1
]}

Gu, Yi ^{[1
]}

Chen, Jiong ^{[1
]}

Guo, Yan ^{[1
]}

Tian, Hengli ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Peoples Hosp 6, Dept Neurosurg, Shanghai 200233, Peoples R China

[2] Chinese Med Hosp Huangdao Dist Jiaonan, Dept Neurosurg, Jiaonan 266406, Shandong, Peoples R China

来源：

ARQUIVOS DE NEURO-PSIQUIATRIA | 2015年 / 73卷 / 04期

关键词：

traumatic brain injury; biomarkers; proteomics; two-dimensional gel electrophoresis; mass spectrometry; PYRUVATE-KINASE M2; ALPHA-II-SPECTRIN; CEREBROSPINAL-FLUID; SERUM BIOMARKERS; PEDIATRIC TBI; PROTEIN; S100B; RESPONSES; HYPOXIA; MODELS;

D O I：

10.1590/0004-282X20150006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Many studies of protein expression after traumatic brain injury (TBI) have identified biomarkers for diagnosing or determining the prognosis of TBI. In this study, we searched for additional protein markers of TBI using a fluid perfusion impact device to model TBI in S-D rats. Two-dimensional gel electrophoresis and mass spectrometry were used to identify differentially expressed proteins. After proteomic analysis, we detected 405 and 371 protein spots within a pH range of 3-10 from sham-treated and contused brain cortex, respectively. Eighty protein spots were differentially expressed in the two groups and 20 of these proteins were identified. This study validated the established biomarkers of TBI and identified potential biomarkers that could be examined in future work.

引用

页码：342 / 349

页数：8

共 29 条

[1] Alpha-II-spectrin after controlled cortical impact in the immature rat brain [J].

Aikman, J. ;

O'Steen, B. ;

Silver, X. ;

Torres, R. ;

Boslaugh, S. ;

Blackband, S. ;

Padgett, K. ;

Wang, K. K. W. ;

Hayes, R. ;

Pineda, J. .

DEVELOPMENTAL NEUROSCIENCE, 2006, 28 (4-5) :457-465

[2] Inhibition of Pyruvate Kinase M2 by Reactive Oxygen Species Contributes to Cellular Antioxidant Responses [J].

Anastasiou, Dimitrios ;

Poulogiannis, George ;

Asara, John M. ;

Boxer, Matthew B. ;

Jiang, Jian-kang ;

Shen, Min ;

Bellinger, Gary ;

Sasaki, Atsuo T. ;

Locasale, Jason W. ;

Auld, Douglas S. ;

Thomas, Craig J. ;

Vander Heiden, Matthew G. ;

Cantley, Lewis C. .

SCIENCE, 2011, 334 (6060) :1278-1283

[3] Altered apoptotic responses in neurons lacking RhoB GTPase [J].

Barberan, Sara ;

McNair, Kara ;

Iqbal, Khalil ;

Smith, Nicola C. ;

Prendergast, George C. ;

Stone, Trevor W. ;

Cobb, Stuart R. ;

Morris, Brian J. .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2011, 34 (11) :1737-1746

[4] Serum S-100B and cleaved-tau are poor predictors of long-term outcome after mild traumatic brain injury [J].

Bazarian, Jeffrey J. ;

Zemlan, Frank P. ;

Mookerjee, Sohug ;

Stigbrand, Torgney .

BRAIN INJURY, 2006, 20 (07) :759-765

[5] MRP9, an unusual truncated member of the ABC transporter superfamily, is highly expressed in breast cancer [J].

Bera, TK ;

Iavarone, C ;

Kumar, V ;

Lee, S ;

Lee, B ;

Pastan, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (10) :6997-7002

[6] Serum Concentrations of Ubiquitin C-Terminal Hydrolase-L1 and αII-Spectrin Breakdown Product 145 kDa Correlate with Outcome after Pediatric TBI [J].

Berger, Rachel P. ;

Hayes, Ronald L. ;

Richichi, Rudolph ;

Beers, Sue R. ;

Wang, Kevin K. W. .

JOURNAL OF NEUROTRAUMA, 2012, 29 (01) :162-167

[7] Serum biomarker concentrations and outcome after pediatric traumatic brain injury [J].

Berger, Rachel Pardes ;

Beers, Sue R. ;

Richichi, Rudolph ;

Wiesman, Daniel ;

Adelson, P. David .

JOURNAL OF NEUROTRAUMA, 2007, 24 (12) :1793-1801

[8] Neuron-specific enolase and S100B in cerebrospinal fluid after severe traumatic brain injury in infants and children [J].

Berger, RP ;

Pierce, MC ;

Wisniewski, SR ;

Adelson, PD ;

Clark, RSB ;

Ruppel, RA ;

Kochanek, PM .

PEDIATRICS, 2002, 109 (02) :E31

[9] Detection of CSF 14-3-3 protein in Guillain-Barre syndrome [J].

Bersano, A. ;

Fiorini, M. ;

Allaria, S. ;

Zanusso, G. ;

Fasoli, E. ;

Gelati, M. ;

Monaco, H. ;

Squintani, G. ;

Monaco, S. ;

Nobile-Orazio, E. .

NEUROLOGY, 2006, 67 (12) :2211-2216

[10] Neuron-Specific Enolase, S100B, and Glial Fibrillary Acidic Protein Levels as Outcome Predictors in Patients With Severe Traumatic Brain Injury [J].

Boehmer, Ana Elisa ;

Oses, Jean Pierre ;

Schmidt, Andre Prato ;

Peron, Cleiton Schweister ;

Krebs, Claudio Liss ;

Oppitz, Paulo Petry ;

D'Avila, Thiago Torres ;

Souza, Diogo Onofre ;

Portela, Luis Valmor ;

Stefani, Marco Antonio .

NEUROSURGERY, 2011, 68 (06) :1624-1630

← 1 2 3 →