Pregnancy complications are associated with oxidative stress induced by accumulation of trophoblastic ROS in the placenta. We employed the human trophoblast HTR8/SVneo cell line to determine the effect of curcumin pre-treatment on H2O2-induced oxidative damage in HTR8/Sveo cells. Cells were pretreated with 2.5 or 5 mu M curcumin for 24 h, and then incubated with 400 mu M H2O2 for another 24 h. The results showed that H2O2 decreased the cell viability and induced excessive accumulation of reactive oxygen species (ROS) in HTR8/Sveo cells. Curcumin pre-treatment effectively protected HTR8/SVneo cells against oxidative stress-induced apoptosis via increasing Bcl-2/Bax ratio and decreasing the protein expression level of cleaved-caspase 3. Moreover, curcumin pre-treatment alleviated the excessive oxidative stress by enhancing the activity of antioxidative enzymes. The antioxidant effect of curcumin was achieved by activating Nrf2 and its downstream antioxidant proteins. In addition, knockdown of Nrf2 by Nrf2-siRNA transfection abolished the protective effects of curcumin on HTR8/SVneo cells against oxidative damage. Taken together, our results show that curcumin could protect HTR8/SVneo cells from H2O2-induced oxidative stress by activating Nrf2 signaling pathway.
机构:
Univ Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Univ Catania, Ctr Res Ocular Pharmacol CERFO, Sch Med, Catania, ItalyUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Bucolo, Claudio
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Drago, Filippo
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Maisto, Rosa
论文数: 0引用数: 0
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Univ Catolica Valencia San Vicente Martir, Fac Med & Odontol, Valencia, SpainUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Maisto, Rosa
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Romano, Giovanni Luca
论文数: 0引用数: 0
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机构:
Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USAUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Romano, Giovanni Luca
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D'Agata, Velia
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Univ Catania, Dept Biomed & Biotechnol Sci, Human Anat & Histol Sect, Via S Sofia 87, I-95123 Catania, ItalyUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
D'Agata, Velia
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Maugeri, Grazia
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Giunta, Salvatore
论文数: 0引用数: 0
h-index: 0
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Univ Catania, Dept Biomed & Biotechnol Sci, Human Anat & Histol Sect, Via S Sofia 87, I-95123 Catania, ItalyUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
机构:
Univ Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Univ Catania, Ctr Res Ocular Pharmacol CERFO, Sch Med, Catania, ItalyUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Bucolo, Claudio
;
论文数: 引用数:
h-index:
机构:
Drago, Filippo
;
Maisto, Rosa
论文数: 0引用数: 0
h-index: 0
机构:
Univ Catolica Valencia San Vicente Martir, Fac Med & Odontol, Valencia, SpainUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Maisto, Rosa
;
Romano, Giovanni Luca
论文数: 0引用数: 0
h-index: 0
机构:
Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Miami, FL 33136 USAUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
Romano, Giovanni Luca
;
D'Agata, Velia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Catania, Dept Biomed & Biotechnol Sci, Human Anat & Histol Sect, Via S Sofia 87, I-95123 Catania, ItalyUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy
D'Agata, Velia
;
论文数: 引用数:
h-index:
机构:
Maugeri, Grazia
;
Giunta, Salvatore
论文数: 0引用数: 0
h-index: 0
机构:
Univ Catania, Dept Biomed & Biotechnol Sci, Human Anat & Histol Sect, Via S Sofia 87, I-95123 Catania, ItalyUniv Catania, Dept Biomed & Biotechnol Sci, Pharmacol Sect, Catania, Italy