Aptamer-antibody dual probes on single-walled carbon nanotube bridged dielectrode: Comparative analysis on human blood clotting factor

被引：24

作者：

Yao, Jian ^{[1
]}

Li, Shujing ^{[2
]}

Zhang, Liping ^{[3
]}

Yang, Yuzhi ^{[3
]}

Gopinath, Subash C. B. ^{[4
,5
]}

Lakshmipriya, Thangavel ^{[5
]}

Zhou, Yawei ^{[6
]}

机构：

[1] Qingdao Univ, Emergency Dept, Affiliated Hosp, Qingdao 266000, Peoples R China

[2] Shandong Univ, Dept Radiol, Jinan Cent Hosp, Jinan 250013, Shandong, Peoples R China

[3] Dezhou Peoples Hosp, Dept Hematol, 1751 Xinhu Rd, Dezhou 253014, Shandong, Peoples R China

[4] Univ Malaysia Perlis, Sch Bioproc Engn, Arau 02600, Malaysia

[5] Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Perlis, Malaysia

[6] Shandong Univ, Dept Hematol, Jinan Cent Hosp, 105 Jiefang Rd, Jinan 250013, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 151卷

关键词：

Haemophilia; Interdigitated sensor; Clotting factors; Single-walled carbon nanotube; Aptamer; FACTOR-IX; GENE-THERAPY; RNA APTAMERS; IDENTIFICATION; COAGULATION; GRAPHENE; BLOCKING; PLATFORM; BINDING;

D O I：

10.1016/j.ijbiomac.2019.10.156

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Haemophilia is a blood clotting disorder known as 'Christmas disease' caused when the blood has defect with the dotting factor(s). Bleeding leads various issues, such as chronic pain, arthritis and a serious complication during the surgery. Identifying this disease is mandatory to take the necessary treatment and maintains the normal clotting. It has been proved that the level of factor IX (FIX) is lesser with haemophilia patient and the attempt here is focused to quantify FIX level by interdigitated electrode (IDE) sensor. Single-walled carbon nanotube (SWCNT) was utilized to modify IDE sensing surface. On this surface, dual probing was evaluated with aptamer and antibody to bring the possible advantages. The detection limit with antibody was found to be 1 pM, while aptamer shows 100 fM. Further, a fine-tuning was attempted with sandwich pattern of aptamer-FIX-antibody and antibody-FIX-aptamer and compared. Specific elevation of detection with 10 folds was noticed and displayed the detection at 100 f. in both sandwich patterns. In addition, FIX was detected in the diluted human serum by aptamer-FIX-antibody sandwich, it was found that FIX detected from the dilution factor 1:640. A novel demonstration is with higher discrimination against other clotting factors, XI and VII. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：1133 / 1138

页数：6

共 5 条

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