A carbohydrate-binding protein, B-GRANULE CONTENT 1, influences starch granule size distribution in a dose-dependent manner in polyploid wheat

被引：40

作者：

Chia, Tansy ^{[1
]}

Chirico, Marcella ^{[1
]}

King, Rob ^{[2
]}

Ramirez-Gonzalez, Ricardo ^{[3
]}

Saccomanno, Benedetta ^{[1
]}

Seung, David ^{[3
]}

Simmonds, James ^{[3
]}

Trick, Martin ^{[3
]}

Uauy, Cristobal ^{[3
]}

Verhoeven, Tamara ^{[3
,4
]}

Trafford, Kay ^{[1
]}

机构：

[1] NIAB, Huntingdon Rd, Cambridge CB3 0LE, England

[2] Rothamsted Res, Harpenden AL5 2JQ, Herts, England

[3] John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England

[4] HAS Univ Appl Sci, NL-5233 DE Shertogenbosch, Netherlands

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2020年 / 71卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Aegilops; B-type starch granule content; crop breeding; FLOURY ENDOSPERM 6; granule size distribution; polymorphous starch; starch granule initiation; TILLING mutant; Triticeae; wheat grain; INITIATION; PHENOTYPE;

D O I：

10.1093/jxb/erz405

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In Triticeae endosperm (e.g. wheat and barley), starch granules have a bimodal size distribution (with A- and B-type granules) whereas in other grasses the endosperm contains starch granules with a unimodal size distribution. Here, we identify the gene, BGC1 (B-GRANULE CONTENT 1), responsible for B-type starch granule content in Aegilops and wheat. Orthologues of this gene are known to influence starch synthesis in diploids such as rice, Arabidopsis, and barley. However, using polyploid Triticeae species, we uncovered a more complex biological role for BGC1 in starch granule initiation: BGC1 represses the initiation of A-granules in early grain development but promotes the initiation of B-granules in mid grain development. We provide evidence that the influence of BGC1 on starch synthesis is dose dependent and show that three very different starch phenotypes are conditioned by the gene dose of BGC1 in polyploid wheat: normal bimodal starch granule morphology; A-granules with few or no B-granules; or polymorphous starch with few normal A- or B-granules. We conclude from this work that BGC1 participates in controlling B-type starch granule initiation in Triticeae endosperm and that its precise effect on granule size and number varies with gene dose and stage of development.

引用

页码：105 / 115

页数：11