Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains

被引:27
|
作者
Literak, I
Rychlik, I
Svobodova, V
Pospisil, Z
机构
[1] Univ Vet & Pharmaceut Sci, Fac Med Vet, Brno 61242, Czech Republic
[2] Vet Res Inst, CS-62132 Brno, Czech Republic
关键词
Toxoplasma gondii; strains; RFLP; virulence; Czech republic;
D O I
10.1016/S0020-7519(98)00090-3
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Restriction fragment length polymorphism analysis of chromosomal DNA from 22 strains of Toxoplasma gondii were characterised using SalI and PstI restriction endonucleases and the TGR1E specific repetitive sequence as a probe. Two virulent strains, RH and P-CZ, had previously been isolated from humans, the remaining 20 strains were isolated from animals in the Czech Republic in 1994 and 1995. Among the 20 recently isolated strains, 19 belonged to an avirulent lineage and only one strain from the wild cat Felis silvestris belonged to a virulent lineage. (C) 1998 Australian Society for Parasitology. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1367 / 1374
页数:8
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