Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis

被引：16

作者：

Aviat, F. ^{[1
,3
]}

Rochereau-Roulet, S. ^{[1
]}

Branger, C. ^{[2
]}

Estavoyer, J. M. ^{[3
]}

Chatrenet, B. ^{[4
]}

Orsonneau, J. L. ^{[5
]}

Thorin, C. ^{[1
]}

Andre-Fontaine, G. ^{[1
]}

机构：

[1] Ecole Natl Vet Nantes, Unite Bacteriol Med & Mol Leptospires, F-44307 Nantes 03, France

[2] Arizona State Univ, Biodesign Inst, Ctr Infect Dis & Vaccinol, Tempe, AZ 85287 USA

[3] Ctr Hosp Univ St Jacques, Serv Malad Infect & Trop, F-25000 Besancon, France

[4] Virbac, F-06511 Carros, France

[5] Ctr Hosp Univ Hotel Dieu, Inst Biol Humaine, F-44000 Nantes, France

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2010年 / 33卷 / 05期

关键词：

Leptospirosis; MAT; ELISA; Hap1; LipL32; Peptide; LINKED-IMMUNOSORBENT-ASSAY; POLYMERASE-CHAIN-REACTION; OUTER-MEMBRANE; DIAGNOSIS; ELISA; ANTIBODY; LIPL32; TESTS;

D O I：

10.1016/j.cimid.2009.01.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Leptospirosis is a worldwide zoonosis. Today, serological diagnosis is generally assessed by MAT. We performed ELISA with a synthetic peptide derived from Hap1/lipL32 which is a protein expressed only by pathogenic Leptospira. Repeatability and thresholds were defined with 85 controls sera and 119 hospitalized leptospirosis. The PP-ELISA repeatability and IgM/IgG cut-off values were based on control sera. For these cut-off values, we observed the IgM-PP-ELISA specificity of 89%, whereas it was 100% for the IgG. Then, we compared PP-ELISA and standard MAT results for leptospirosis patients. The concordance rate for IgM-PP-ELISA and MAT was low (43%), whereas it was 85% for IgG-PP-ELISA and MAT. During the first 5 days after hospitalization, PP-ELISA gave positive results in 13 out of 16 patients (81%) whereas 8 out of 14 patients (57%) were positive to MAT. ELISA using Hap1/lipL32-derived synthetic peptide PP is an earlier serological diagnosis of human leptospirosis than MAT. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：375 / 387

页数：13

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