Cloning and functional analysis of the VcCXIP4 and VcYSL6 genes as Cd-regulating genes in blueberry

Blueberries (Vaccinium ssp.) show relatively high resistance to pollution and have been reported to successfully colonize acid and heavy metal-contaminated soils. Blueberries were subjected to cadmium stress using a simulated pot-culture method. The intact CDS regions of VcCXIP4 and VcYSL6 were obtained, VcCXIP4 was located in the nucleus, while VcYSL6 was located in the chloroplast. Both genes were constructed into a modified plant expression vector pCambia1301 for tobacco transformation with agrobacterium infection methods. Results showed that VcCXIP4 did not function alone in regulating cadmium (Cd) transport. Cd content of Cd in the leaves of VcYSL6 transgenic tobacco by 15.57% under high Cd concentration. Both, VcCXIP4 and VcYSL6 genes were up-regulated under Cd stress. Blueberry primarily accumulated excess Cd in the root, but Cd content in the fruit was almost independent of Cd content in the soil. Further, the effect of soil Cd content on fruit Cd content was not significant. VcCXIP4 is likely to interact with other proteins to regulate excess Cd in blueberry, while VcYSL6 is a Cd transporter required for excess Cd detoxification in blueberry.