Loss of methylation of Snrpn in postovulatory aging mouse oocyte

被引：60

作者：

Liang, Xing-Wei ^{[1
,2
]}

Zhu, Jia-Qiao ^{[1
]}

Miao, Yi-Liang ^{[1
]}

Liu, Jing-He ^{[1
]}

Wei, Liang ^{[1
]}

Lu, Sheng-Sheng ^{[2
]}

Hou, Yi ^{[1
]}

Schatten, Heide ^{[3
]}

Lu, Ke-Huan ^{[2
]}

Sun, Qing-Yuan ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

[2] Guangxi Univ, Guangxi Lab Subtrop Bioresource Conservat & Utili, Anim Reprod Inst, Nanning 530004, Peoples R China

[3] Univ Missouri, Dept Vet Pathol, Columbia, MO 65211 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DNA methylation; imprinting; aging; mouse oocyte;

D O I：

10.1016/j.bbrc.2008.03.105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prolonged residence Of postovulatory oocyte in the oviduct or prolonged culture in vitro can lead to oocyte aging, which significantly affects pre- and post-implantation embryo development. In this study, we employed bisulfite sequencing and COBRA methods to investigate the DNA methylation status of differentially methylated regions (DMRs) of Snrpn and Peg1/Mest, two maternally imprinted genes, in postovulatory oocytes aged in vivo and in vitro. The results showed that Snrpn DMR was clearly demethylated in oocytes aged in vivo at 29 h post-hCG and in denuded oocytes aged in vitro for the same time period. However, Peg1/Mest did not show any demethylation in all aged groups at 29 h post-hCG. These data indicate that oocytes undergo time-dependent demethylation of Snrpn DMR during the process of postovulatory aging. (c) 2008 Elsevier Inc. All rights reserved

引用

页码：16 / 21

页数：6

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