Loss of methylation of Snrpn in postovulatory aging mouse oocyte

被引：58
作者：
Liang, Xing-Wei [1 ,2 ]
Zhu, Jia-Qiao [1 ]
Miao, Yi-Liang [1 ]
Liu, Jing-He [1 ]
Wei, Liang [1 ]
Lu, Sheng-Sheng [2 ]
Hou, Yi [1 ]
Schatten, Heide [3 ]
Lu, Ke-Huan [2 ]
Sun, Qing-Yuan [1 ]
机构：
[1] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
[2] Guangxi Univ, Guangxi Lab Subtrop Bioresource Conservat & Utili, Anim Reprod Inst, Nanning 530004, Peoples R China
[3] Univ Missouri, Dept Vet Pathol, Columbia, MO 65211 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 01期
基金：
中国国家自然科学基金;
关键词：
DNA methylation; imprinting; aging; mouse oocyte;
D O I：
10.1016/j.bbrc.2008.03.105
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Prolonged residence Of postovulatory oocyte in the oviduct or prolonged culture in vitro can lead to oocyte aging, which significantly affects pre- and post-implantation embryo development. In this study, we employed bisulfite sequencing and COBRA methods to investigate the DNA methylation status of differentially methylated regions (DMRs) of Snrpn and Peg1/Mest, two maternally imprinted genes, in postovulatory oocytes aged in vivo and in vitro. The results showed that Snrpn DMR was clearly demethylated in oocytes aged in vivo at 29 h post-hCG and in denuded oocytes aged in vitro for the same time period. However, Peg1/Mest did not show any demethylation in all aged groups at 29 h post-hCG. These data indicate that oocytes undergo time-dependent demethylation of Snrpn DMR during the process of postovulatory aging. (c) 2008 Elsevier Inc. All rights reserved
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页码：16 / 21
页数：6
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