Gene profiling of laser-microdissected brain regions and sub-regions

被引：13
作者：
Sanna, PP [1 ]
King, AR [1 ]
van der Stap, LD [1 ]
Repunte-Canonigo, V [1 ]
机构：
[1] Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA
来源：
BRAIN RESEARCH PROTOCOLS | 2005年 / 15卷 / 02期
关键词：
laser microdissection; laser capture microdissection; laser pressure catapulting; microarray; array; gene profiling; expression; mRNA; proteomics; RAE230A; rat; 230A; 230B; Rat Genome 230 2.0; 230; 2.0; RNU34; Rat Neurobiology U34; mouse genome 430 2.0; mouse expression set 430; murine 11K set; MG_U74; MOE430; Mu1; lk;
D O I：
10.1016/j.brainresprot.2005.04.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The application of transcriptomics and proteomics approaches to accurately dissected anatomically-defined brain regions and sub-regions remains a central focus of current neurobiological investigations as well as a necessary step towards single-neuron neurogenomics and neuroproteomics. A protocol is described for the simple, rapid, and reproducible laser microdissection of brain regions and sub-regions for microarray-based gene expression analyses from individual rats or mice using two rounds of in vitro transcription (IVT). The results presented also demonstrate that the current Affymetrix GeneChip(R) arrays are well suited for this experimental design with high reproducibility and limited effects of the shortening of target RNA caused by the double IVT approach. (C) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：66 / 74
页数：9
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