Reversible thrombin detection by aptamer functionalized STING sensors

被引：52

作者：

Actis, Paolo ^{[1
,2
,3
,4
]}

Rogers, Adam ^{[1
]}

Nivala, Jeff ^{[1
]}

Vilozny, Boaz ^{[1
,3
,4
]}

Seger, R. Adam ^{[1
,3
,4
]}

Jejelowo, Olufisayo ^{[2
]}

Pourmand, Nader ^{[1
,3
,4
]}

机构：

[1] Univ Calif Santa Cruz, Dept Biomol Engn, Santa Cruz, CA 95064 USA

[2] Texas So Univ, Dept Biol, Houston, TX 77004 USA

[3] UC Santa Cruz, Adv Studies Labs, Moffett Field, CA 94035 USA

[4] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 11期

基金：

美国国家卫生研究院; 美国国家航空航天局;

关键词：

Nanopipette; Label-free; Nanopore; Aptamer; Thrombin; STING; CURRENT RECTIFICATION; NANOPIPETTE; MOLECULES; NANOPORES; SELECTION; LIGANDS; BIND;

D O I：

10.1016/j.bios.2011.05.010

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Signal Transduction by Ion NanoGating (STING) is a label-free technology based on functionalized quartz nanopipettes. The nanopipette pore can be decorated with a variety of recognition elements and the molecular interaction is transduced via a simple electrochemical system. A STING sensor can be easily and reproducibly fabricated and tailored at the bench starting from inexpensive quartz capillaries. The analytical application of this new biosensing platform, however, was limited due to the difficult correlation between the measured ionic current and the analyte concentration in solution. Here we show that STING sensors functionalized with aptamers allow the quantitative detection of thrombin. The binding of thrombin generates a signal that can be directly correlated to its concentration in the bulk solution. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：4503 / 4507

页数：5

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