Detection of TMV with ODA-H2O2-HRP voltammetric enzyme-linked immunoassay system

被引：13

作者：

Jiao, K

Zhang, SS

Wei, L

Liu, CF

Zhang, CL

Zhang, ZF

Liu, JY

Wei, P

机构：

[1] Qingdao Inst Chem Technol, Dept Appl Chem, Qingdao 266042, Peoples R China

[2] Minist Agr, Inst Plant Quarantine, Beijing 100029, Peoples R China

[3] Beijing Inst Geol, Beijing 100029, Peoples R China

来源：

TALANTA | 1998年 / 47卷 / 05期

基金：

中国国家自然科学基金;

关键词：

tobacco mosaic virus (TMV); horseradish peroxidase (HRP); o-dianisidine; electrochemical immunoassay; voltammetry;

D O I：

10.1016/S0039-9140(98)00192-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

o-Dianisidine (ODA)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay system has firstly been used for the detection of tobacco mosaic virus (TMV). HRP catalyzes strongly the oxidation reaction of ODA by H2O2, the product of which produces a sensitive second order derivative linear sweep voltammetric peak at potential of -0.56 V (versus SCE) in Britton-Robinson (BR) buffer. HRP activity has been measured with this voltammetric peak and TMV detected through immunoreaction. The detection limit for HRP is 9.25 x 10(-7) mU l(-1) and the linear range is 2.5 x 10(-6)-5.0 x 10(-4) mU l(-1). The detection limit for the clarified TMV is 0.25 ng ml(-1) and the highest dilution ratio detected for the infected leaf sap is 1:8 x 10(5). The sensitivity for TMV detection with this method is higher than that with the enzyme-linked immunosorbent spectrophotometric assay (ELISA) using ODA-H2O2-HRP system. The processes of the enzyme-catalyzed reaction and the electro-reduction of the product of the enzyme-catalyzed reaction have been described. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：1129 / 1137

页数：9

共 14 条

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