Simultaneous Determination of Food-Related Biogenic Amines and Precursor Amino Acids Using in Situ Derivatization Ultrasound-Assisted Dispersive Liquid-Liquid Microextraction by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

被引：38

作者：

He, Yongrui ^{[1
,2
]}

Zhao, Xian-En ^{[1
,2
]}

Wang, Renjun ^{[1
,2
]}

Wei, Na ^{[1
,2
]}

Sun, Jing ^{[3
,4
]}

Dang, Jun ^{[3
,4
]}

Chen, Guang ^{[1
,2
]}

Liu, Zhiqiang ^{[5
,6
]}

Zhu, Shuyun ^{[1
,2
]}

You, Jinmao ^{[1
,2
,3
,4
]}

机构：

[1] Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China

[2] Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China

[3] Chinese Acad Sci, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China

[4] Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China

[5] Chinese Acad Sci, Natl Ctr Mass Spectrometry Changchun, 5625 Renmin St, Changchun 130022, Peoples R China

[6] Chinese Acad Sci, Key Lab Tradit Chinese Med Chem & Mass Spectromet, Changchun Inst Appl Chem, 5625 Renmin St, Changchun 130022, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2016年 / 64卷 / 43期

基金：

中国国家自然科学基金;

关键词：

in situ derivatization; ultrasound-assisted dispersive liquid-liquid microextraction; amino acids; biogenic amines; UHPLC-MS/MS (MRM); WINE SAMPLES; PRECOLUMN DERIVATIZATION; FLUORESCENCE DETECTION; EXTRACTION; HPLC; BEER; NEUROTRANSMITTERS; BENZOYLATION; QUANTITATION; POLYAMINES;

D O I：

10.1021/acs.jafc.6b03536

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A simple, rapid, sensitive, selective, and environmentally friendly method; based on in situ derivatization ultrasound-assisted dispersive liquid-liquid microextraction (in situ DUADLLME) coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) using multiple reaction monitoring (MRM) mode has been developed for the simultaneous determination of food-related biogenic amines and amino acids. A new mass-spectrometry sensitive derivatization reagent 4'-carbonyl chloride rosamine (CCR) was designed, synthesized, and first reported. Parameters and conditions of in situ DUADLLME and UHPLC-MS/MS were, optimized in detail. Under the optimized conditions, the in situ DUADLLME was completed speedily (within 1 min) with high derivatization efficiencies (>= 98.5%). With the cleanup and concentration of microextraction step, good analytical performance was obtained for the analytes. The results showed that this method was accurate and practical for quantification of biogenic amines and amino acids in common food samples (red wine, beer, wine, cheese, sausage, and fish).

引用

页码：8225 / 8234

页数：10

共 38 条

[1] A novel dispersive liquid-liquid microextraction (DLLME) gas chromatography-mass spectrometry (GC-MS) method for the determination of eighteen biogenic amines in beer [J].