Development of an enzyme-linked immunosorbent assay for detection of ofloxacin residue in environment matrix

被引：0

作者：

Hou, Xiaolin ^{[1
]}

Guo, Kaijun ^{[1
]}

Bao, Jie ^{[2
]}

Wu, Yongnin ^{[3
]}

Li, Huanrong ^{[1
]}

机构：

[1] China Agr Univ, Dept Anim Sci, Beijing 102206, Peoples R China

[2] Beijing Anim Hlth Inspect Inst, Beijing 100044, Peoples R China

[3] Chinese Ctr Dis Control & Prevent, Inst Nutr & Food Hyg, Beijing 100050, Peoples R China

来源：

JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT | 2011年 / 9卷 / 3-4期

关键词：

Ofloxacin; ELISA; wastewater; manure; sludge; TANDEM MASS-SPECTROMETRY; INDIRECT COMPETITIVE ELISA; LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; PHASE EXTRACTION; RAW-MILK; ANTIBIOTICS; FLUOROQUINOLONES; CIPROFLOXACIN; ENROFLOXACIN;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ofloxacin polyclonal antibody was prepared and cross-reactivity to other fluroquinones was observed. Further, a rapid, low-cost ELISA analytical technique was developed, with IC(50) at 1.2 ng.mL(-1), for the analysis of ofloxacin in the environment matrix including wastewater, manure and sludge. The mean recoveries and coefficients of variation were in a range of 74-83% and 8.5-12.2%, respectively, in wastewater. The mean recoveries and coefficients of variation in pig manure and sludge were from 62% to 78% and from 8.4% to 14.4%, respectively. Concentrations of ofloxacin using ELISA were sometimes greater than measured with LC-MS/MS. It may be due to cross-reactivity to structurally similar compounds or metabolites. Results from this study showed that the ELISA can be used as a preliminary screening tool for later instrumental analysis.

引用

页码：779 / 783

页数：5

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