Regulated expression of putative membrane progestin receptor homologues in human endometrium and gestational tissues

被引:112
作者
Fernandes, MS
Pierron, V
Michalovich, D
Astle, S
Thornton, S
Peltoketo, H
Lam, EWF
Gellersen, B
Huhtaniemi, I
Allen, J
Brosens, JJ [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Inst Reprod & Dev Biol, Wolfson & Weston Res Ctr Family Hlth, London W12 0NN, England
[2] Inpharmat Ltd, London Biosci Innovat Ctr, London NW1 0NH, England
[3] Univ Warwick, Inst Biomed Res, Warwick Med Sch, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
[4] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Dept Canc Med, Canc Res UK Labs, London W12 0NN, England
[5] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Dept Canc Med, Sect canc Cell Biol, London W12 0NN, England
[6] Endokrinologikum Hamburg, D-20251 Hamburg, Germany
关键词
D O I
10.1677/joe.1.06242
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Rapid non-genomic actions of progesterone are implicated in many aspects of female reproduction. Recently, three human homologues of the fish membrane progestin receptor (mPR) have been identified. We combined bioinformatic analysis with expression profiling to define further the role of these mPRs in human reproductive tissues. Sequence analysis confirmed that the mPRs belong to a larger, highly conserved family of proteins, termed 'progestin and adiponectin receptors' (PAQRs). A comparison of the expression of mPR, transcripts with that of two related PAQR family members, PAQRIII and PAQRIX, in cycling endometrium and pregnancy tissues revealed markedly divergent expression levels and profiles. For instance. endometrial expression of mPR alpha and gamma and PAQRIX was cycle-dependent whereas the onset of parturition was associated with a marked reduction in myometrial mPR alpha and beta transcripts. Interestingly, mPR alpha and PAQRIX were most highly expressed in the placenta, and the tissue expression levels of both genes correlated inversely with that of the nuclear PR. Phylogenetic analysis demonstrated that PAQRIX belongs to the mPR subgroup of proteins. We also validated a polyclonal antibody raised against the carboxy-terminus of human mPRa. Immunohistochemical analysis demonstrated more intense immunoreactivity in placental syncytiotrophoblasts than in endometrial glands or stroma. The data suggest important functional roles for mPR alpha, and possibly PAQRIX, in specific reproductive tissues, particularly those that express low levels of nuclear PR.
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页码:89 / 101
页数:13
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