Effects of γ-hexachlorocyclohexane on apoptosis induced by serum deprivation in PC12 cells

被引:4
作者
Aoki, Kentaro [4 ]
Egawa, Mari [4 ]
Saito, Takeshi [2 ]
Hosokawa, Toshiyuki [3 ]
Kurasaki, Masaaki [1 ,4 ]
机构
[1] Hokkaido Univ, Fac Environm Earth Sci, Sapporo, Hokkaido 0600810, Japan
[2] Hokkaido Univ, Sch Med, Dept Hlth Sci, Sapporo, Hokkaido 0600810, Japan
[3] Hokkaido Univ, Ctr R&D Higher Educ, Sapporo, Hokkaido 0600810, Japan
[4] Hokkaido Univ, Grad Sch Environm Sci, Sapporo, Hokkaido 0600810, Japan
关键词
apoptosis; gamma-HCH; PC12; cell; caspase; cytochrome C; Bcl-2; family;
D O I
10.1080/03601230802174573
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The effects of -hexachlorocyclohexane, one of the endocrine disrupters, widely used for agricultural and medicinal purpose, on apoptosis in PC12 cells were investigated using western blotting analysis and reverse transcriptase-DNA polymerase chain reaction (RT-PCR) method. Apoptosis is a fundamental process necessary for development of individuals and organs. Although -HCH at high concentration did not have an effect on cell viability and apoptosis, DNA fragmentation was slightly enhanced, and apoptotic factors; Bax, Bad, cytochrome c and caspase-3 showed tendency to increase by the addition of a low dose of -HCH to the cell medium. However these changes were not statistically significant. It was concluded that -HCH did not affect on apoptosis in the PC12 cell line system, although -HCH has been reported to induce apoptosis in other cell lines.
引用
收藏
页码:471 / 475
页数:5
相关论文
共 50 条
[21]   BCL-2-related protein expression in apoptosis: Oxidative stress versus serum deprivation in PC12 cells [J].
Maroto, R ;
PerezPolo, JR .
JOURNAL OF NEUROCHEMISTRY, 1997, 69 (02) :514-523
[22]   Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury [J].
Bakhtiari, Elham ;
Hosseini, Azar ;
Mousavi, Seyed Hadi .
AVICENNA JOURNAL OF PHYTOMEDICINE, 2015, 5 (03) :231-237
[23]   Estradiol protects PC12 cells against CoCl2-induced apoptosis [J].
Jung, Ji Yeon ;
Roh, Kwang Hoon ;
Jeong, Yeon Jin ;
Kim, Sun Hun ;
Lee, Eun Ju ;
Kim, Min Seok ;
Oh, Won Mann ;
Oh, Hee Kyun ;
Kim, Won Jae .
BRAIN RESEARCH BULLETIN, 2008, 76 (06) :579-585
[24]   Ceramide induces apoptosis in PC12 cells [J].
Hartfield, PJ ;
Mayne, GC ;
Murray, AW .
FEBS LETTERS, 1997, 401 (2-3) :148-152
[25]   Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death [J].
Kantati, Yendube T. ;
Kodjo, Magloire K. ;
Lefranc, Benjamin ;
Eklu-Gadegbeku, Kwashie ;
Leprince, Jerome ;
Vaudry, David .
IBRO NEUROSCIENCE REPORTS, 2025, 19 :110-116
[26]   Protective Effects of Phillyrin on H2O2-induced Oxidative Stress and Apoptosis in PC12 Cells [J].
Wei, Teng ;
Tian, Wulin ;
Yan, Haiyang ;
Shao, Guoxi ;
Xie, Guanghong .
CELLULAR AND MOLECULAR NEUROBIOLOGY, 2014, 34 (08) :1165-1173
[27]   Flow cytometric analysis of serum deprivation-induced apoptosis of PC12 cells, with special reference to role of bcl-2 [J].
Satoh, T ;
Sakai, N ;
Kubo, T ;
Enokido, Y ;
Uchiyama, Y ;
Hatanaka, H .
NEUROSCIENCE LETTERS, 1995, 201 (02) :119-122
[28]   Cyclosporine A- and FK506-induced apoptosis in PC12 cells [J].
Takadera, T. ;
Sakamoto, Y. ;
Hizume, Y. ;
Ohyashiki, T. .
CELL BIOLOGY AND TOXICOLOGY, 2007, 23 (05) :355-360
[29]   Neuroprotective effects of Total Saikosaponins of Bupleurum yinchowense on corticosterone-induced apoptosis in PC12 cells [J].
Li, Zong-Yang ;
Guo, Zhi ;
Liu, Ya-Min ;
Liu, Xin-Min ;
Chang, Qi ;
Liao, Yong-hong ;
Pan, Rui-Le .
JOURNAL OF ETHNOPHARMACOLOGY, 2013, 148 (03) :794-803
[30]   Protective effects of aloin on oxygen and glucose deprivation-induced injury in PC12 cells [J].
Chang, Renyuan ;
Zhou, Ru ;
Qi, Xue ;
Wang, Jing ;
Wu, Fan ;
Yang, Wenli ;
Zhang, Wannian ;
Sun, Tao ;
Li, Yuxiang ;
Yu, Jianqiang .
BRAIN RESEARCH BULLETIN, 2016, 121 :75-83