Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines

被引:26
|
作者
Petrillo, Caroline Pereira [1 ]
Carneiro, Newton Portilho [2 ]
Corsetti Purcino, Antonio Alvaro [2 ]
Siqueira Carvalho, Carlos Henrique [3 ]
Alves, Jose Donizeti [1 ]
Carneiro, Andrea Almeida [2 ]
机构
[1] Univ Fed Lavras, Dept Fisiol Vegetal, BR-37200000 Lavras, MG, Brazil
[2] Embrapa Milho & Sorgo, BR-35701970 Sete Lagoas, MG, Brazil
[3] Embrapa Cafe, Varginha, MG, Brazil
关键词
Zea mays; ammonium glufosinate; beta-glucuronidase; immature embryos; phosphinotricin acetyl transferase; plant transformation;
D O I
10.1590/S0100-204X2008000300012
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by Southern blot analysis as well as bar and uidA gene expressions. The maize genetic transformation protocol developed in this work will possibly improve the efficiency to produce new transgenic tropical maize lines expressing desirable agronomic characteristics.
引用
收藏
页码:371 / 378
页数:8
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